Cloning and characterization of an apoptosis-related DNA fragmentation factor (DFF) from oyster, Crassostrea hongkongensis

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	Cloning and characterization of an apoptosis-related DNA fragmentation factor (DFF) from oyster, Crassostrea hongkongensis
	Xiang, ZM ; Qu, FF ; Qi, L ; Ying, T ; Li, J ; Shu, X ; Yu, ZN
刊名	FISH & SHELLFISH IMMUNOLOGY
	2014
卷号	38 期号:1 页码:119-126
关键词	Crassostrea hongkongensis Immunity Apoptosis ChDFFA
ISSN号	1050-4648
通讯作者	carlzyu@scsio.ac.cn
中文摘要	Apoptosis plays an important pathophysiological role in the homeostasis of immune systems. DNA fragmentation factors (DFFs) have been shown to be essential for DNA fragmentation, and the resultant DNA fragments follow a laddering pattern during apoptosis in vertebrates. In invertebrates, the functions of the DFF orthologs are not well characterized; therefore, we cloned and characterized a bivalve DFFA ortholog from the Hong Kong oyster Crassostrea hongkongensis (designated ChDFFA). The full-length cDNA of ChDFFA is 1186 bp in length and encodes a putative protein of 200 amino acids that contains an N-terminal CAD domain and a DFF-C domain at its C-terminus. Real-time RT-PCR results showed that ChDFFA is ubiquitously expressed in several tissues, and its highest expression is in gill. Following a 3- to 48-h challenge by microbial infection, the expression of ChDFFA increased in hemocytes. Using fluorescence microscopy, ChDFFA was localized in nuclei when exogenously expressed in HeLa cells. In addition, overexpression of ChDFFA inhibited the transcriptional activities of p53/p21-Luc reporter genes in HEK293T cells. These results suggest that ChDFFA may be involved in immune response reactions in the Hong Kong oyster C hongkongensis. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
资助信息	National Science Foundation of China [U1201215, 31202021]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095]
原文出处	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000336353700016
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10445]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Xiang, ZM,Qu, FF,Qi, L,et al. Cloning and characterization of an apoptosis-related DNA fragmentation factor (DFF) from oyster, Crassostrea hongkongensis[J]. FISH & SHELLFISH IMMUNOLOGY,2014,38(1):119-126.
APA	Xiang, ZM.,Qu, FF.,Qi, L.,Ying, T.,Li, J.,...&Yu, ZN.(2014).Cloning and characterization of an apoptosis-related DNA fragmentation factor (DFF) from oyster, Crassostrea hongkongensis.FISH & SHELLFISH IMMUNOLOGY,38(1),119-126.
MLA	Xiang, ZM,et al."Cloning and characterization of an apoptosis-related DNA fragmentation factor (DFF) from oyster, Crassostrea hongkongensis".FISH & SHELLFISH IMMUNOLOGY 38.1(2014):119-126.