Effect of temperature on gene expression in the pearl oyster Pinctada fucata

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Effect of temperature on gene expression in the pearl oyster Pinctada fucata
	Liu, WG ; Huang, XD ; Lin, JS ; He, MX
刊名	JOURNAL OF OCEAN UNIVERSITY OF CHINA
	2014
卷号	13 期号:3 页码:509-515
关键词	seawater temperature heat shock protein gene expression pattern Pinctada fucata
ISSN号	1672-5182
通讯作者	hmx@scsio.ac.cn
中文摘要	In this study, we examined the effect of elevated temperature on the expression patterns of genes, i.e., nacrein, irr, n16, n19, and hsp70 in the pearl oyster Pinctada fucata. The experiment was carried out at 4 temperatures, i.e., 20A degrees C (ambient, control), 24, 28A degrees C, and 32A degrees C. The expression levels of target genes in P. fucata were assayed at 0, 6, 24, 48, and 96 h via real-time polymerase chain reaction. Results showed that the expression levels of nacrein and irr had no significant variations among different time points below 28A degrees C, but significantly increased over time at 32A degrees C. The expression levels of n16 and n19 did not change markedly at 20A degrees C. The former increased significantly at 6 h and 24 h while the latter substantially decreased during 6-96 h at 24, 28 and 32A degrees C. Among different temperatures, the level of n16 was significantly lower at 20A degrees C than at other temperatures during 6-96 h, and the level of n19 significantly varied among different temperatures at 48 h and 96 h. The expression level of hsp70 was significantly higher at 32A degrees C than at 20, 24 and 28A degrees C at 24 h. These results demonstrated that elevated temperature impacted the physiological processes of P. fucata and potentially influenced its adaptability to thermal stress.
学科主题	Oceanography
收录类别	SCI
资助信息	National Natural Science Foundation of China [41006090]; Joint Program of NSFC-Guangdong [U0831001]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21]
原文出处	OCEAN UNIV CHINA
WOS记录号	WOS:000335672600023
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10436]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Liu, WG,Huang, XD,Lin, JS,et al. Effect of temperature on gene expression in the pearl oyster Pinctada fucata[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2014,13(3):509-515.
APA	Liu, WG,Huang, XD,Lin, JS,&He, MX.(2014).Effect of temperature on gene expression in the pearl oyster Pinctada fucata.JOURNAL OF OCEAN UNIVERSITY OF CHINA,13(3),509-515.
MLA	Liu, WG,et al."Effect of temperature on gene expression in the pearl oyster Pinctada fucata".JOURNAL OF OCEAN UNIVERSITY OF CHINA 13.3(2014):509-515.