The first molluscan acute phase serum amyloid A (A-SAA) identified from oyster Crassostrea hongkongensis: Molecular cloning and functional characterization

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	The first molluscan acute phase serum amyloid A (A-SAA) identified from oyster Crassostrea hongkongensis: Molecular cloning and functional characterization
	Qu, FF ; Xiang, ZM ; Yu, ZN
刊名	FISH & SHELLFISH IMMUNOLOGY
	2014
卷号	39 期号:2 页码:145-151
关键词	Crassostrea hongkongensis Acute phase serum amyloid A (A-SAA) Innate immunity NF-kappa B
ISSN号	1050-4648
通讯作者	carlzyu@scsio.ac.cn
中文摘要	Serum amyloid A (SAA), a major evolutionarily conserved acute-phase protein, participates in many biological processes in eukaryotic cells, including innate immunity. However, little information regarding the relationship between SAA and innate immunity in mollusks is currently available. In this report, the first bivalve SAA (referred to as ChSAA) gene was identified and characterized from the Hong Kong oyster Crassostrea hongkongensis. Its full-length cDNA is 623 bp, including a 5'-UTR of 147 bp, a 3'-UTR of 56 bp containing a poly(A) tail and an open reading frame (ORF) of 420 bp that encodes a polypeptide of 139 amino acids. The predicted amino acid sequence of ChSAA comprises characteristic motifs of the SAA family, including a typical signal peptide and a conserved SAA domain. Comparison and phylogenetic analyses suggested that ChSAA shares a high identity to known acute-phase SAA proteins (A-SAAs). In addition, quantitative real-time PCR analysis revealed that ChSAA is constitutively expressed in all tissues examined, with the highest expression level in the mantle, and that its expression was acutely and significantly up-regulated in hemocytes following challenge by Vibrio alginolyticus (G(-)), Staphylococcus haemolyticus (G(+)) or Saccharomyces cerevisiae (fungus). Furthermore, over-expression of ChSAA via transfection with a ChSAA expression vector led to significantly increased NF-kappa B activity in HEK293T cells. These results suggest that ChSAA is likely to constitute a member of the A-SAA family involved in anti-pathogen responses in C hongkongensis. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
资助信息	National Natural Science Foundation of China [31202021]; National Science Foundation of China [41176150]; National Basic Research Program of China [2010CB126404]
原文出处	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000339773000004
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10415]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Qu, FF,Xiang, ZM,Yu, ZN. The first molluscan acute phase serum amyloid A (A-SAA) identified from oyster Crassostrea hongkongensis: Molecular cloning and functional characterization[J]. FISH & SHELLFISH IMMUNOLOGY,2014,39(2):145-151.
APA	Qu, FF,Xiang, ZM,&Yu, ZN.(2014).The first molluscan acute phase serum amyloid A (A-SAA) identified from oyster Crassostrea hongkongensis: Molecular cloning and functional characterization.FISH & SHELLFISH IMMUNOLOGY,39(2),145-151.
MLA	Qu, FF,et al."The first molluscan acute phase serum amyloid A (A-SAA) identified from oyster Crassostrea hongkongensis: Molecular cloning and functional characterization".FISH & SHELLFISH IMMUNOLOGY 39.2(2014):145-151.