Phage M13Ko7 Detection With Biosensor Based On Imaging Ellipsometry And Afm Microscopic Confirmation

	Phage M13Ko7 Detection With Biosensor Based On Imaging Ellipsometry And Afm Microscopic Confirmation
	Qi C(齐财); Lin Y; Feng J(冯静); Wang ZH(王战会); Zhu CF; Meng YH(孟永宏); Yan XY; Wan LJ; Jin G(靳刚); Jin G
刊名	Virus Research
	2009
通讯作者邮箱	gajin@imech.ac.cn
卷号	140 期号:1-2 页码:79-84
关键词	Phage Detection Imaging Ellipsometry Microarray Atomic Force Microscope Microfluidic Surface Modification Atomic-Force Microscopy Neutralizing Antibody Sars Coronavirus Silicon Surface Protein Immunosensor Visualization Epitope System Pcr
ISSN号	0168-1702
通讯作者	Jin G
中文摘要	A rapid detection and identification of pathogens is important for minimizing transfer and spread of disease. A label-free and multiplex biosensor based on imaging ellipsometry (BIE) had been developed for the detection of phage M13KO7. The surface of silicon wafer is modified with aldehyde, and proteins can be patterned homogeneously and simultaneously on the surface of silicon wafer in an array format by a microfluidic system. Avidin is immobilized on the surface for biotin-anti-M13 immobilization by means of interaction between avidin and biotin, which will serve as ligand against phage M13KO7. Phages M13KO7 are specifically captured by the ligand when phage M13KO7 solution passes over the surface, resulting in a significant increase of mass surface concentration of the anti-M13 binding phage M13KO7 layer, which could be detected by imaging ellipsometry with a sensitivity of 10(9) pfu/ml. Moreover, atomic force microscopy is also used to confirm the fact that phage M13KO7 has been directly captured by ligands on the surface. It indicates that BIE is competent for direct detection of phage M13KO7 and has potential in the field of virus detection. (C) 2008 Elsevier B.V. All rights reserved.
类目[WOS]	Virology
研究领域[WOS]	Virology
关键词[WOS]	ATOMIC-FORCE MICROSCOPY ; NEUTRALIZING ANTIBODY ; SARS CORONAVIRUS ; SILICON SURFACE ; PROTEIN ; IMMUNOSENSOR ; VISUALIZATION ; EPITOPE ; SYSTEM ; PCR
收录类别	SCI
语种	英语
WOS记录号	WOS:000264650600011
公开日期	2009-08-03 ; 2009-10-09
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/26710]
专题	力学研究所_国家微重力实验室
通讯作者	Jin G
推荐引用方式 GB/T 7714	Qi C,Lin Y,Feng J,et al. Phage M13Ko7 Detection With Biosensor Based On Imaging Ellipsometry And Afm Microscopic Confirmation[J]. Virus Research,2009,140(1-2):79-84.
APA	齐财.,Lin Y.,冯静.,王战会.,Zhu CF.,...&Jin G.(2009).Phage M13Ko7 Detection With Biosensor Based On Imaging Ellipsometry And Afm Microscopic Confirmation.Virus Research,140(1-2),79-84.
MLA	齐财,et al."Phage M13Ko7 Detection With Biosensor Based On Imaging Ellipsometry And Afm Microscopic Confirmation".Virus Research 140.1-2(2009):79-84.