Characterization of dissolved organic matter using high-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) with a multiple wavelength absorbance detector

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	Characterization of dissolved organic matter using high-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) with a multiple wavelength absorbance detector
	Yan, Mingquan ; Korshin, Gregory ; Wang, Dongsheng ; Cai, Zhenxiao
刊名	CHEMOSPHERE
	2012
卷号	87 期号:8 页码:879－885
关键词	HPLC-SEC Multiple wavelength absorbance detector Molecular weight DOM
ISSN号	0045-6535
中文摘要	High-performance liquid chromatography-size exclusion chromatography (HPLC-SEC) coupled with a multiple wavelength absorbance detector (200-445 nm) was used in this study to investigate the apparent molecular weight (AMW) distributions of dissolved organic matter (DOM). Standard DOM, namely humic acid, fulvic acid and hydrophilic acid, from the Suwannee River were tested to ascertain the performance and sensitivity of the method. In addition to four compounds groups: humic substances (Peak 1, AMW 16 kD), fulvic acids (Peak 2, AMW 11 kD), low AMW acids (Peak 3, AMW 5 kD), and low AMW neutral and amphiphilic molecules, proteins and their amino acid building blocks (Peak 4, AMW 3 kD), an new group that appears to include low AMW, 6-10 kD, humic substances was found based on investigating the spectra at various elution times. The spectroscopic parameter S->365 (slope at wavelengths >365 nm) was determined to be a good predictor of the AMW of the DOM. The detector wavelength played an important role in evaluating the AMW distribution. For some fractions, such as the humic and low AMW non-aromatic substances, the error in measurement was +/- 30% as determined by two-dimensional chromatograms detected at an artificially selected wavelength. HPLC-SEC with multiple wavelength absorbance detection was found to be a useful technique for DOM characterization. It characterized the AMW distributions of DOM more accurately and provided additional, potentially important information concerning the properties of DOM with varying AMWs. (C) 2012 Elsevier Ltd. All rights reserved.
WOS记录号	WOS:000302988100008
公开日期	2014-11-04
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/7827]
专题	生态环境研究中心_环境水质学国家重点实验室
推荐引用方式 GB/T 7714	Yan, Mingquan,Korshin, Gregory,Wang, Dongsheng,et al. Characterization of dissolved organic matter using high-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) with a multiple wavelength absorbance detector[J]. CHEMOSPHERE,2012,87(8):879－885.
APA	Yan, Mingquan,Korshin, Gregory,Wang, Dongsheng,&Cai, Zhenxiao.(2012).Characterization of dissolved organic matter using high-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) with a multiple wavelength absorbance detector.CHEMOSPHERE,87(8),879－885.
MLA	Yan, Mingquan,et al."Characterization of dissolved organic matter using high-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) with a multiple wavelength absorbance detector".CHEMOSPHERE 87.8(2012):879－885.