Quantum dots impair macrophagic morphology and the ability of phagocytosis by inhibiting the Rho-associated kinase signaling

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Quantum dots impair macrophagic morphology and the ability of phagocytosis by inhibiting the Rho-associated kinase signaling
	Qu, Guangbo ; Zhang, Changwen ; Yuan, Lin ; He, Jiuyang ; Wang, Zhe ; Wang, Lixin ; Liu, Sijin ; Jiang, Guibin
刊名	Chemistry; Science & Technology - Other Topics; Materials Science; Physics
	2012
卷号	4 期号:7 页码:2239-2244
ISSN号	2040-3364
中文摘要	Quantum dots (QDs) are fluorescent semiconductor nanoparticles that have broad excitation spectra, narrow emission peaks, long fluorescence lifetimes, and the ability to easily conjugate with bio-molecules. Due to these distinct characteristics, QDs represent promising substances in biological imaging and labelling. However, the side and adverse effects of QDs are also widely studied. Herein, we recognize macrophages as the pivotal cells in ingesting QDs, and that the accumulation of QDs inside macrophages leads to significant morphological alterations and a remarkable reduction of their ability to erythrophagocytize in vitro. In a mouse model with chronic exposure to QDs, red blood cell (RBC) retention in spleens and severe splenomegaly were observed, presumably due to attenuated macrophagic erythrophagocytosis in vivo. Importantly, we demonstrated that QDs greatly inhibited the Rho-associated kinase (ROCK) activity, resulting in impaired fidelity of the actin cytoskeleton and actin-rich structure (such as surface protrusions), which was assumed to be the molecular basis underlying the blunted macrophagic morphology and reduced ability to phagocytize. The combined data provide insights into QDs' intracellular trafficking, localization and biological fate in macrophages, and the resultant impairment to cytoskeleton coupled with inhibition on the ROCK signalling would decrease the macrophagic ability to erythrophagocytize with diminished RBC recycling and splenic RBC retention in animals.
WOS记录号	WOS:000301591300005
公开日期	2014-11-27
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/8075]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Qu, Guangbo,Zhang, Changwen,Yuan, Lin,et al. Quantum dots impair macrophagic morphology and the ability of phagocytosis by inhibiting the Rho-associated kinase signaling[J]. Chemistry; Science & Technology - Other Topics; Materials Science; Physics,2012,4(7):2239-2244.
APA	Qu, Guangbo.,Zhang, Changwen.,Yuan, Lin.,He, Jiuyang.,Wang, Zhe.,...&Jiang, Guibin.(2012).Quantum dots impair macrophagic morphology and the ability of phagocytosis by inhibiting the Rho-associated kinase signaling.Chemistry; Science & Technology - Other Topics; Materials Science; Physics,4(7),2239-2244.
MLA	Qu, Guangbo,et al."Quantum dots impair macrophagic morphology and the ability of phagocytosis by inhibiting the Rho-associated kinase signaling".Chemistry; Science & Technology - Other Topics; Materials Science; Physics 4.7(2012):2239-2244.