QQ客服 官方微博 反馈留言

	Hydrophilic organic/salt-containing aqueous two-phase solvent system for counter-current chromatography: A novel technique for separation of polar compounds
	Liu, Dan1; Zou, Xiaowei1; Gao, Mingzhe1; Gu, Ming2; Xiao, Hongbin1,3
刊名	JOURNAL OF CHROMATOGRAPHY A
	2014-08-22
卷号	1356期号:aug页码:157-162
关键词	Hydrophilic organic/salt-containing aqueous two-phase system High-speed counter-current chromatography Partition coefficient Elution mode
ISSN号	0021-9673
其他题名	J. Chromatogr. A
中文摘要	Hydrophilic organic/salt-containing aqueous two-phase system composing of ethanol, water and ammonium sulfate for separation polar compounds was investigated on multilayer coil associated with J-type HSCCC devices. Compared to the classical polar solvent system based on 1-butanol-water or PEG1000-ammonium sulfate-water, the water content of upper phase in ethanol-ammonium sulfate-water systems was from 53.7% to 32.8% (wt%), closed to PEG1000-ammonium sulfate-water aqueous two-phase systems and higher than 1-butanol-water (22.0%, wt%). Therefore, the polarity of ethanol-ammonium sulfate-water is in the middle of 1-butanol-water and PEG-ammonium sulfate-water system, which is quite good for separating polar compounds like phenols, nucleosides and amino acids with low partition coefficient in 1-octanol-water system. The retention of stationary phase in four elution mode on type-J counter-current chromatography devices with multilayer coil column changed from 26% to 71%. Hydrodynamic trend possess both intermediate and hydrophilic solvent system property, which closely related to the composition of solvent system. The applicability of this system was demonstrated by successful separation of adenosine, uridine guanosine and cytidine. (C) 2014 Elsevier B.V. All rights reserved.
英文摘要	Hydrophilic organic/salt-containing aqueous two-phase system composing of ethanol, water and ammonium sulfate for separation polar compounds was investigated on multilayer coil associated with J-type HSCCC devices. Compared to the classical polar solvent system based on 1-butanol-water or PEG1000-ammonium sulfate-water, the water content of upper phase in ethanol-ammonium sulfate-water systems was from 53.7% to 32.8% (wt%), closed to PEG1000-ammonium sulfate-water aqueous two-phase systems and higher than 1-butanol-water (22.0%, wt%). Therefore, the polarity of ethanol-ammonium sulfate-water is in the middle of 1-butanol-water and PEG-ammonium sulfate-water system, which is quite good for separating polar compounds like phenols, nucleosides and amino acids with low partition coefficient in 1-octanol-water system. The retention of stationary phase in four elution mode on type-J counter-current chromatography devices with multilayer coil column changed from 26% to 71%. Hydrodynamic trend possess both intermediate and hydrophilic solvent system property, which closely related to the composition of solvent system. The applicability of this system was demonstrated by successful separation of adenosine, uridine guanosine and cytidine. (C) 2014 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	SALVIA-MILTIORRHIZA BUNGE ; FERMENTATION BROTHS ; CHLOROGENIC ACID ; QUINIC ACID ; PURIFICATION ; EXTRACTION ; PHASE ; 1,3-PROPANEDIOL ; ANTIOXIDANTS ; DERIVATIVES
收录类别	SCI
原文出处	://WOS:000340302600016
语种	英语
WOS记录号	WOS:000340302600016
公开日期	2014-09-30
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/11465]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 3.Chinese Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Dan,Zou, Xiaowei,Gao, Mingzhe,et al. Hydrophilic organic/salt-containing aqueous two-phase solvent system for counter-current chromatography: A novel technique for separation of polar compounds[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1356(aug):157-162.
APA	Liu, Dan,Zou, Xiaowei,Gao, Mingzhe,Gu, Ming,&Xiao, Hongbin.(2014).Hydrophilic organic/salt-containing aqueous two-phase solvent system for counter-current chromatography: A novel technique for separation of polar compounds.JOURNAL OF CHROMATOGRAPHY A,1356(aug),157-162.
MLA	Liu, Dan,et al."Hydrophilic organic/salt-containing aqueous two-phase solvent system for counter-current chromatography: A novel technique for separation of polar compounds".JOURNAL OF CHROMATOGRAPHY A 1356.aug(2014):157-162.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。