Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices

	Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices
	Zhou, Mengying 1; Ma, Huipeng 2,3; Lin, Hongli 1; Qin, Jianhua 2
刊名	biomaterials
	2014-02-01
卷号	35 期号:5 页码:1390-1401
关键词	Biomimetic of proximal tubule Epithelial-to-mesenchymal transition Microfluidic Migration C3a
ISSN号	0142-9612
通讯作者	hongli lin ; 秦建华
产权排序	待补充
合作状况	英
英文摘要	in proteinuric nephropathy, epithelial-to-mesenchymal transition (emt) is an important mechanism that causes renal interstitial fibrosis. the precise role of emt in the pathogenesis of fibrosis remains controversial, partly due to the absence of suitable in vitro or in vivo models. we developed two microfluidic and compartmental chips that reproduced the fluidic and three-dimensional microenvironment of proximal tubular epithelial cells in vivo. using one microfluidic device, we stimulated epithelial cells with a flow of healthy human serum, heat-inactivated serum and complement c3a, which mimicked the flow of urine within the proximal tubule. we observed that epithelial cells exposed to serum proteins became apoptotic or developed a mesenchymal phenotype. incubating cells with c3a induced similar features. however, cells exposed to heat-inactivated serum did not adopt the mesenchymal phenotype. furthermore, we successfully recorded the cellular morphological changes and the process of transmigration into basement membrane extract during emt in real-time using another three-dimensional microdevice. in conclusion, we have established a cell-culture system that mimics the native microenvironment of the proximal tubule to a certain extent. our data indicates that emt did occur in epithelial cells that were exposed to serum proteins, and c3a plays an essential role in this pathological process. (c) 2013 elsevier ltd. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; technology
类目[WOS]	engineering, biomedical ; materials science, biomaterials
研究领域[WOS]	engineering ; materials science
关键词[WOS]	fibrosis ; c3a ; complement ; kidney ; nephropathy ; fibroblasts ; expression
收录类别	SCI
资助信息	2，2
原文出处	1401
语种	英语
WOS记录号	WOS:000330156100005
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119808]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Dalian Med Univ, Affiliated Hosp 1, Dept Nephrol, Dalian 116011, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 3.Dalian Med Univ, Coll Med Lab, Dalian 116044, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Mengying,Ma, Huipeng,Lin, Hongli,et al. Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices[J]. biomaterials,2014,35(5):1390-1401.
APA	Zhou, Mengying,Ma, Huipeng,Lin, Hongli,&Qin, Jianhua.(2014).Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices.biomaterials,35(5),1390-1401.
MLA	Zhou, Mengying,et al."Induction of epithelial-to-mesenchymal transition in proximal tubular epithelial cells on microfluidic devices".biomaterials 35.5(2014):1390-1401.