Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE-Cys column

	Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE-Cys column
	Wei, Jie 1; Shen, Aijin 2; Wan, Huihui 3; Yan, Jingyu 2; Yang, Bingcheng 1; Guo, Zhimou 2; Zhang, Feifang 1; Liang, Xinmiao 1,2
刊名	journal of separation science
	2014-07-01
卷号	37 期号:14 页码:1781-1787
关键词	Aminoglycosides Hydrophilic interaction chromatography Separation selectivity Zwitterionic stationary phases
ISSN号	1615-9306
通讯作者	郭志谋
产权排序	待补充
合作状况	英
英文摘要	hydrophilic interaction liquid chromatography has emerged as a valuable alternative approach to ion-pair chromatography for the separation of aminoglycoside antibiotics in recent years. however, the resolution of structurally related aminoglycosides is a great challenge owing to the limited selectivity. in this work, a cysteine-based zwitterionic stationary phase (named click te-cys) was utilized and compared with five commonly used hydrophilic interaction liquid chromatography columns. click te-cys displayed much better selectivity for structurally similar aminoglycosides. the retention behaviors of aminoglycosides were investigated in detail, revealing that low ph (2.7 or 3.0) and high buffer concentration (>= 50 mm) were preferable for achieving good peak shape and selectivity. effective resolution of ten aminoglycosides including spectinomycin, dihydrostreptomycin, streptomycin, gentamicin c1, gentamicin c2/c2a, gentamicin c1a, kanamycin, paromonycin, tobramycin, and neomycin was realized at optimized conditions. additionally, spectinomycin and its related impurities were successfully resolved. the results indicated the great potential of the click te-cys column in the separation of aminoglycoside mixtures and related impurities.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	tandem-mass-spectrometry ; hydrophilic interaction chromatography ; performance liquid-chromatography ; light-scattering detection ; stationary-phase ; confirmatory method ; hplc-elsd ; residues ; dihydrostreptomycin ; streptomycin
收录类别	SCI
资助信息	2，2
原文出处	1787
语种	英语
WOS记录号	WOS:000339669700009
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119711]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 3.Dalian Univ Technol, Chem Anal & Res Ctr, Fac Chem Environm & Biol Sci & Technol, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Wei, Jie,Shen, Aijin,Wan, Huihui,et al. Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE-Cys column[J]. journal of separation science,2014,37(14):1781-1787.
APA	Wei, Jie.,Shen, Aijin.,Wan, Huihui.,Yan, Jingyu.,Yang, Bingcheng.,...&Liang, Xinmiao.(2014).Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE-Cys column.journal of separation science,37(14),1781-1787.
MLA	Wei, Jie,et al."Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE-Cys column".journal of separation science 37.14(2014):1781-1787.