A simple microfluidic strategy for cell migration assay in an in vitro wound-healing model

	A simple microfluidic strategy for cell migration assay in an in vitro wound-healing model
	Zhang, Min 1; Li, Hongjing 2; Ma, Huipeng 3; Qin, Jianhua 1
刊名	wound repair and regeneration
	2013-11-01
卷号	21 期号:6 页码:897-903
ISSN号	1067-1927
通讯作者	秦建华
产权排序	待补充
合作状况	英
英文摘要	in vitro scratch wound assays are commonly used strategies to measure cell repair rate, facilitating the study of cell migration, tissue reorganization, and cell division. this work presented a simple and novel microfluidic device that allowed a quantitative investigation of the cell migration and cell proliferation behaviors in an in vitro wound-healing model, especially focused on the scratch assay. the microfluidic device is composed of four units, which include cell growth regions and cell-free regions created by micropillars. using this device, we evaluated the proliferation and migration process of human gastric epithelial cells in the presence of different concentrations of the epidermal growth factor, and investigated the migration behavior of mesenchymal stem cells toward tumor cells as well. this approach has the unique capability to create localized cell-free regions in parallel, and facilitate quantitative research on cell migration in the wound-healing process, providing a powerful platform for elucidating the mechanism of cell migration in regeneration medicine.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	cell biology ; dermatology ; medicine, research & experimental ; surgery
研究领域[WOS]	cell biology ; dermatology ; research & experimental medicine ; surgery
关键词[WOS]	motility ; complex
收录类别	SCI
资助信息	1，1
原文出处	903
语种	英语
WOS记录号	WOS:000326327100162
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119682]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 1, Dept Orthoped, Dalian, Liaoning, Peoples R China 3.Dalian Med Univ, Coll Med Lab, Dalian, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Min,Li, Hongjing,Ma, Huipeng,et al. A simple microfluidic strategy for cell migration assay in an in vitro wound-healing model[J]. wound repair and regeneration,2013,21(6):897-903.
APA	Zhang, Min,Li, Hongjing,Ma, Huipeng,&Qin, Jianhua.(2013).A simple microfluidic strategy for cell migration assay in an in vitro wound-healing model.wound repair and regeneration,21(6),897-903.
MLA	Zhang, Min,et al."A simple microfluidic strategy for cell migration assay in an in vitro wound-healing model".wound repair and regeneration 21.6(2013):897-903.