Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions

	Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions
	Yang, F.1; Mao, J.1; He, X. W.1; Chen, L. X.1; Zhang, Y. K.1,2
刊名	analytical and bioanalytical chemistry
	2013-08-01
卷号	405 期号:21 页码:6639-6648
关键词	Boronate affinity chromatography Hybrid monolith Glycoprotein Physiological pH Separation
通讯作者	陈朗星 ; 张玉奎
产权排序	待补充
合作状况	英
英文摘要	in this study, a boronate-silica hybrid affinity monolith was prepared for specific capture of glycoproteins at neutral ph condition. the monolith was synthesized via a facile one-pot procedure in a stainless steel column by concurrently mixing hydrolyzed alkoxysilanes tetramethoxysilane and vinyltrimethoxysilane, organic monomer 3-acrylamidophenylboronic acid and initiator 2,2'-azobisisobutyronitrile together. the polycondensation of alkoxysilanes and copolymerization of organic monomer and vinyl-silica monolith were carried out successively by reacting at different temperatures. after optimizing the preparation conditions, the resulting hybrid affinity monolith was systematically characterized and exhibited excellent affinity to both cis-diol-containing small molecules and glycoproteins at neutral and physiological ph, including adenosine, horseradish peroxidase, transferrin and ovalbumin. the binding capacity of ovalbumin on monolith was measured to be 2.5 mg g(-1) at ph 7.0. furthermore, the hybrid affinity monolith was applied to the separation of transferrin from bovine serum sample at a physiological condition. good repeatability was obtained and the relative standard deviations of retention time were 1.15 and 4.77 % (n = 5) for run-to-run and column-to-column, respectively.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	capillary liquid-chromatography ; maldi-tof ms ; cis-diol ; mass-spectrometry ; column ; glycopeptides ; enrichment ; glycosylation ; separation ; electrochromatography
收录类别	SCI
资助信息	2，5
原文出处	6648
语种	英语
WOS记录号	WOS:000322705600004
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119677]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Nankai Univ, State Key Lab Med Chem Biol, Dept Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Yang, F.,Mao, J.,He, X. W.,et al. Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions[J]. analytical and bioanalytical chemistry,2013,405(21):6639-6648.
APA	Yang, F.,Mao, J.,He, X. W.,Chen, L. X.,&Zhang, Y. K..(2013).Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions.analytical and bioanalytical chemistry,405(21),6639-6648.
MLA	Yang, F.,et al."Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions".analytical and bioanalytical chemistry 405.21(2013):6639-6648.