Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique

	Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique
	Li, Qinran 1,2; Yang, Kaiguang 1; Liu, Jinxiang 1,2; Zhang, Lihua 1; Liang, Zhen 1; Zhang, Yukui 1
刊名	microchimica acta
	2013-11-01
卷号	180 期号:15-16 页码:1379-1386
关键词	Hierarchical imprinting Protein imprinted polymers Transferrin. Selective recognition
通讯作者	张丽华
产权排序	待补充
合作状况	英
英文摘要	a novel kind of transferrin imprinted polymer particles was synthesized by a hierarchical strategy. first, transferrin was immobilized on silica beads by non-covalent absorption. then, a pre-polymerization mixture, composed of 1,4-bis(acryloyl)piperazine, methacrylamide, methacrylic acid, ammonium sulfate and polyoxyethylene sorbitan monolaurate, was irrigated into the pores of silica particles, and polymerized at 25 a degrees c. finally, the silica matrix was etched with ammonium hydrogen fluoride, not only to remove the template protein, but also to expose protein recognition sites on the surface of the imprinted polymer. the binding capacity of the transferrin-imprinted particles is 6.3 mg of protein per gram of material, and the time required to reach adsorption equilibrium was less than 10 min. the imprinting factor of transferrin is ca. 3.3 in the presence of ribonuclease b, cytochrome c and beta-lactoglobulin. the results indicate that these imprinted polymer particles can recognize transferrin with good selectivity, high binding capacity and fast mass transfer. they may be applied as an artificial antibody to remove the high abundance proteins in plasma.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	electrostatic functional-groups ; enzyme-inhibitors ; ribonuclease-a ; polymers ; polyacrylamide ; plasma ; proteomics ; hydrogel ; beads ; sds
收录类别	SCI
资助信息	1，1
原文出处	1386
语种	英语
WOS记录号	WOS:000326803800004
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119109]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, Qinran,Yang, Kaiguang,Liu, Jinxiang,et al. Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique[J]. microchimica acta,2013,180(15-16):1379-1386.
APA	Li, Qinran,Yang, Kaiguang,Liu, Jinxiang,Zhang, Lihua,Liang, Zhen,&Zhang, Yukui.(2013).Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique.microchimica acta,180(15-16),1379-1386.
MLA	Li, Qinran,et al."Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique".microchimica acta 180.15-16(2013):1379-1386.