Enzymatic Hydrolysis of Penicillin in an Integrated System Containing Ionic Liquids

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 研究所（批量导入）

	Enzymatic Hydrolysis of Penicillin in an Integrated System Containing Ionic Liquids
	Jiang, Y. Y.; Guo, C.; Xia, H. S.; Liu, H. Z.
	2010
会议名称	236th National Meeting of the American-Chemical-Society
会议日期	AUG 17-21, 2008
会议地点	Philadelphia, PA
关键词	POTASSIUM PHOSPHATE SOLUTIONS ACYLASE-CATALYZED SYNTHESIS BETA-LACTAM ANTIBIOTICS 6-AMINOPENICILLANIC ACID POLY(ETHYLENE GLYCOL) ORGANIC COSOLVENTS WATER ACTIVITY SALT-SOLUTIONS LOW PH EQUILIBRIUM
其他题名	ACS Symp. Ser.
页码	77-89
中文摘要	Ionic liquids were employed as novel green solvents to develop an integrated process involving ionic liquids aqueous two-phase system (ILATPS) and mixed ionic liquids/water two-phase system (MILWS) for separation and enzymatic catalysis of penicillin. First, hydrophilic [C(4)mim]BF4 (1-butyl-3-methylimidazolium tetrafluoroborate) and NaH2PO4 salt form an ionic liquid aqueous two-phase system (ILATPS), which could extract penicillin from its fermentation broth efficiently. Second, hydrophobic [C(4)mim]PF6 (1-butyl-3-methylimidazolium hexafluorophosphate) is introduced into the ionic liquid-rich phase of ILATPS containing penicillin and converses it into MILWS. Penicillin is hydrolyzed by penicillin acylase in the water phase of MILWS at pH 5. The byproduct phenylacetic acid (PAA) is partitioned into the ionic liquids mixture phase, while the intended product 6-aminopenicillanic acid (6-APA) is precipitated at this pH. In comparison with a similar butyl acetate/water system (BAWS) at pH 4, MILWS exhibits two advantages: (1) The selectivity between PAA and penicillin is greatly optimized at pH 5 by varying the mole ratio of [C(4)mim]PF6/[C(4)mim]BF4 in MILWS, whereas in BAWS the unalterable nature of the organic solvent restricts the optimized pH for maximum selectivity between PAA and penicillin at pH 4. (2) The pH for 6-APA precipitation in BAWS is 4, whereas it shifts to pH 5 in MILWS due to the complexation between negatively charged 6-APA and the cationic surface of the ionic liquids micelle. As a result, the removal of the two products from the enzyme sphere at relatively high pH is permitted in MILWS, which is beneficial for enzymatic activity and stability in comparison with the acidic pH 4 environment in BAWS.
收录类别	ISTP
会议网址	://WOS:000292522900007
会议录	ACS Symposium Series 卷: 1038 页: 77-89
会议录出版者	Amer Chemical Soc
语种	英语
内容类型	会议论文
源URL	[http://ir.ipe.ac.cn/handle/122111/11133]
专题	过程工程研究所_研究所（批量导入）
推荐引用方式 GB/T 7714	Jiang, Y. Y.,Guo, C.,Xia, H. S.,et al. Enzymatic Hydrolysis of Penicillin in an Integrated System Containing Ionic Liquids[C]. 见:236th National Meeting of the American-Chemical-Society. Philadelphia, PA. AUG 17-21, 2008.://WOS:000292522900007.