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	Hydrophilic modification gigaporous resins with poly(ethylenimine) for high-throughput proteins ion-exchange chromatography
	Zhang, Rongyue; Li, Qiang; Gao, Yang; Li, Juan; Huang, Yongdong; Song, Cui; Zhou, Weiqing; Ma, Guanghui; Su, Zhiguo
刊名	JOURNAL OF CHROMATOGRAPHY A
	2014-05-23
卷号	1343期号:1页码:109-118
关键词	Hydrophilicity Gigaporous microspheres Anion-exchange medium Branched polyethyleneimine High-throughput
ISSN号	0021-9673
其他题名	J. Chromatogr. A
中文摘要	High hydrophilicity of gigaporous microspheres based on a copolymer of poly(glycidyl methacrylate)-co-divinyl benzene (PGMA-DVB) was successfully realized through coating the branched polyethyleneimine (PEI) in PGMA-DVB microspheres. PEI with various molecules weights and different branching agents were identified in terms of protein recovery as evaluation approach. For this evaluation, PEI600 (M-w = 600) and poly (ethylene glycol) diglycidyl ether (PEGDE, M-w = 400) were used as modification agent and branching agent, respectively. The modified microspheres showed good permeability and revealed a certain mechanical strength. After modification, the protein recovery increased from 40% to >90%. The protein recovery increased with the branched generations and the first and second generations could give the protein recovery of 93% and 96%, respectively. Meanwhile, the ionic capacity also showed a rising trend in the range of 0.11-0.32 mmol/mL with the branched generations. But the dynamic binding capacity of protein (bovine serum albumin, BSA as the model protein) increased at first and then decreased. Analysis of the dry microspheres structure by mercury intrusion method as well as observation of the branched PEI on PGMA-DVB membrane in aqueous solution indicated that excess PEI chains with the extended state in the second generation would block the small pores and decrease the accessible surface area. Therefore, the protein capacity on the second generation, on the contrary, was lower than that on the first generation. Meanwhile, it was found that the PEI chains in the modified microspheres changed their construction from the extended to the collapsed state with increase of NaCl concentration. And the corresponding pore size of the modified microspheres increased with salt concentration through low-field nuclear magnetic resonance. Dynamic binding capacity of proteins on the modified supports did not significantly change with increase of the flow rate. The media showed good performance for separation three model proteins at high flow rate of 1084 cm/h. This modified gigaporous microspheres had a large potential in application for rapid separation of biomolecules. (C) 2014 Elsevier B.V. All rights reserved.
英文摘要	High hydrophilicity of gigaporous microspheres based on a copolymer of poly(glycidyl methacrylate)-co-divinyl benzene (PGMA-DVB) was successfully realized through coating the branched polyethyleneimine (PEI) in PGMA-DVB microspheres. PEI with various molecules weights and different branching agents were identified in terms of protein recovery as evaluation approach. For this evaluation, PEI600 (M-w = 600) and poly (ethylene glycol) diglycidyl ether (PEGDE, M-w = 400) were used as modification agent and branching agent, respectively. The modified microspheres showed good permeability and revealed a certain mechanical strength. After modification, the protein recovery increased from 40% to >90%. The protein recovery increased with the branched generations and the first and second generations could give the protein recovery of 93% and 96%, respectively. Meanwhile, the ionic capacity also showed a rising trend in the range of 0.11-0.32 mmol/mL with the branched generations. But the dynamic binding capacity of protein (bovine serum albumin, BSA as the model protein) increased at first and then decreased. Analysis of the dry microspheres structure by mercury intrusion method as well as observation of the branched PEI on PGMA-DVB membrane in aqueous solution indicated that excess PEI chains with the extended state in the second generation would block the small pores and decrease the accessible surface area. Therefore, the protein capacity on the second generation, on the contrary, was lower than that on the first generation. Meanwhile, it was found that the PEI chains in the modified microspheres changed their construction from the extended to the collapsed state with increase of NaCl concentration. And the corresponding pore size of the modified microspheres increased with salt concentration through low-field nuclear magnetic resonance. Dynamic binding capacity of proteins on the modified supports did not significantly change with increase of the flow rate. The media showed good performance for separation three model proteins at high flow rate of 1084 cm/h. This modified gigaporous microspheres had a large potential in application for rapid separation of biomolecules. (C) 2014 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	TRANSFER RADICAL POLYMERIZATION ; MICELLES SWELLING METHOD ; HIGH-CAPACITY ; CAPILLARY ELECTROCHROMATOGRAPHY ; POLY(GLYCIDYL METHACRYLATE) ; PERFUSION CHROMATOGRAPHY ; POLYSTYRENE MICROSPHERES ; LIQUID-CHROMATOGRAPHY ; STATIONARY PHASES ; RAPID SEPARATION
收录类别	SCI
原文出处	://WOS:000336195800014
语种	英语
WOS记录号	WOS:000336195800014
公开日期	2014-08-28
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/11032]
专题	过程工程研究所_研究所（批量导入）
作者单位	Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Rongyue,Li, Qiang,Gao, Yang,et al. Hydrophilic modification gigaporous resins with poly(ethylenimine) for high-throughput proteins ion-exchange chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1343(1):109-118.
APA	Zhang, Rongyue.,Li, Qiang.,Gao, Yang.,Li, Juan.,Huang, Yongdong.,...&Su, Zhiguo.(2014).Hydrophilic modification gigaporous resins with poly(ethylenimine) for high-throughput proteins ion-exchange chromatography.JOURNAL OF CHROMATOGRAPHY A,1343(1),109-118.
MLA	Zhang, Rongyue,et al."Hydrophilic modification gigaporous resins with poly(ethylenimine) for high-throughput proteins ion-exchange chromatography".JOURNAL OF CHROMATOGRAPHY A 1343.1(2014):109-118.

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