Inhibition of methemoglobin formation in aqueous solutions under aerobic conditions by the addition of amino acids

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	Inhibition of methemoglobin formation in aqueous solutions under aerobic conditions by the addition of amino acids
	Wei, Yuping 1,2; Li, Chunlong 1,2; Zhang, Liang 1,2; Su, Zhiguo 1; Xu, Xia 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2014-03-01
卷号	64 期号:1 页码:267-275
关键词	Amino acid Hemoglobin Methemoglobin Molecular simulation Stability
ISSN号	0141-8130
其他题名	Int. J. Biol. Macromol.
中文摘要	Hemoglobin (Hb) as an important iron-containing oxygen-transport protein is easily oxidized to the ferric met-form, methemoglobin (metHb), and loses the capacity of binding oxygen during storage. In this study, the experimental data indicate that the presence of Tyr and Glu significantly suppress the metHb formation in the Hb solutions in aqueous environment under aerobic conditions at the temperature of 25 and 37 C, respectively. At pO(2) of 144 Torr the metHb percentage in the Hb solutions was the lowest with less than 10% at day 7 after incubation with Tyr at the ratio of 24 at pH 9.5 at 25 C. At 37 C, the metHb percentage did not reach 5% after 12 h of incubation with Glu at the ratio of 24 at pH 9. Molecular simulation analysis suggest that the presence of Tyr or Glu may contribute to the formation of the breakwater network, the stabilization of distal histidine, the changes in the size of heme pocket, and eventually result in the inhibition of metHb formation. This study provides insight into a new design for Hb-oxygen based carriers with strongly inhibition of metHb formation in aqueous environment under aerobic conditions, even at physiological temperature in vitro. (C) 2013 Elsevier B.V. All rights reserved.
英文摘要	Hemoglobin (Hb) as an important iron-containing oxygen-transport protein is easily oxidized to the ferric met-form, methemoglobin (metHb), and loses the capacity of binding oxygen during storage. In this study, the experimental data indicate that the presence of Tyr and Glu significantly suppress the metHb formation in the Hb solutions in aqueous environment under aerobic conditions at the temperature of 25 and 37 C, respectively. At pO(2) of 144 Torr the metHb percentage in the Hb solutions was the lowest with less than 10% at day 7 after incubation with Tyr at the ratio of 24 at pH 9.5 at 25 C. At 37 C, the metHb percentage did not reach 5% after 12 h of incubation with Glu at the ratio of 24 at pH 9. Molecular simulation analysis suggest that the presence of Tyr or Glu may contribute to the formation of the breakwater network, the stabilization of distal histidine, the changes in the size of heme pocket, and eventually result in the inhibition of metHb formation. This study provides insight into a new design for Hb-oxygen based carriers with strongly inhibition of metHb formation in aqueous environment under aerobic conditions, even at physiological temperature in vitro. (C) 2013 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	HEMOGLOBIN-VESICLES ; OXYGEN CARRIERS ; IN-VIVO ; MOLECULAR-MECHANISM ; LIPID-PEROXIDATION ; CIRCULAR-DICHROISM ; BLOOD SUBSTITUTES ; HB VESICLES ; AUTOXIDATION ; REDUCTION
收录类别	SCI
原文出处	://WOS:000334147300040
语种	英语
WOS记录号	WOS:000334147300040
公开日期	2014-08-28
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/10991]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wei, Yuping,Li, Chunlong,Zhang, Liang,et al. Inhibition of methemoglobin formation in aqueous solutions under aerobic conditions by the addition of amino acids[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2014,64(1):267-275.
APA	Wei, Yuping,Li, Chunlong,Zhang, Liang,Su, Zhiguo,&Xu, Xia.(2014).Inhibition of methemoglobin formation in aqueous solutions under aerobic conditions by the addition of amino acids.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,64(1),267-275.
MLA	Wei, Yuping,et al."Inhibition of methemoglobin formation in aqueous solutions under aerobic conditions by the addition of amino acids".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 64.1(2014):267-275.