Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes

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	Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes
	Gong, Wen-Bing 1,2; Liu, Wei 2,3; Lu, Ying-Ying 2,4; Bian, Yin-Bing 1,2; Zhou, Yan 1,2; Kwan, Hoi Shan 5; Cheung, Man Kit 5; Xiao, Yang 1,2
刊名	FUNGAL BIOLOGY
	2014-03-01
卷号	118 期号:3 页码:295-308
关键词	Genetic linkage map Quantitative trait loci Shiitake mushroom Vegetative mycelium growth rate
ISSN号	1878-6146
通讯作者	Xiao, Y (reprint author), Huazhong Agr Univ, Key Lab Agromicrobial Resource & Dev, Minist Agr, Wuhan 430070, Hubei Province, Peoples R China.
中文摘要	The most saturated linkage map for Lentinula edodes to date was constructed based on a mono-. karyotic population of 146 single spore isolates (SSIs) using sequence-related amplified polymorphism (SRAP), target region amplification polymorphism (TRAP), insertion deletion (InDel) markers, and the mating-type loci. Five hundred and twenty-four markers were located on 13 linkage groups (LGs). The map spanned a total length of 1006.1 cM, with an average marker spacing of 2.0 cM. Quantitative trait loci (QTLs) mapping was utilized to uncover the loci regulating and controlling the vegetative mycelium growth rate on various synthetic media, and complex medium for commercial cultivation of L. edodes. Two and 13 putative QTLs, identified respectively in the monokaryotic population and two testcross dikaryotic populations, were mapped on seven different LGs. Several vegetative mycelium growth rate-related QTLs uncovered here were clustered on LG4 (Qmgr1, Qdgr1, Qdgr2 and Qdgr9) and LG6 (Qdgr3, Qdgr4 and Qdgr5), implying the presence of main genomic areas responsible for growth rate regulation and control. The QTL hotspot region on LG4 was found to be in close proximity to:the region containing the mating-type A (MAT-A) locus. Moreover, Qdgr2 on LG4 was detected on different media, contributing 8.07%-23.71% of the phenotypic variation. The present study provides essential information for QTL mapping and marker-assisted selection (MAS) in L. edodes. (C) 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
英文摘要	The most saturated linkage map for Lentinula edodes to date was constructed based on a mono-. karyotic population of 146 single spore isolates (SSIs) using sequence-related amplified polymorphism (SRAP), target region amplification polymorphism (TRAP), insertion deletion (InDel) markers, and the mating-type loci. Five hundred and twenty-four markers were located on 13 linkage groups (LGs). The map spanned a total length of 1006.1 cM, with an average marker spacing of 2.0 cM. Quantitative trait loci (QTLs) mapping was utilized to uncover the loci regulating and controlling the vegetative mycelium growth rate on various synthetic media, and complex medium for commercial cultivation of L. edodes. Two and 13 putative QTLs, identified respectively in the monokaryotic population and two testcross dikaryotic populations, were mapped on seven different LGs. Several vegetative mycelium growth rate-related QTLs uncovered here were clustered on LG4 (Qmgr1, Qdgr1, Qdgr2 and Qdgr9) and LG6 (Qdgr3, Qdgr4 and Qdgr5), implying the presence of main genomic areas responsible for growth rate regulation and control. The QTL hotspot region on LG4 was found to be in close proximity to:the region containing the mating-type A (MAT-A) locus. Moreover, Qdgr2 on LG4 was detected on different media, contributing 8.07%-23.71% of the phenotypic variation. The present study provides essential information for QTL mapping and marker-assisted selection (MAS) in L. edodes. (C) 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Mycology
研究领域[WOS]	Mycology
关键词[WOS]	BASIDIOMYCETE PLEUROTUS-OSTREATUS ; AGARICUS-BISPORUS ; MATING-TYPE ; AFLP MARKERS ; POLYMORPHISM MARKERS ; SHIITAKE MUSHROOM ; CAP COLOR ; IDENTIFICATION ; FUNGUS ; REGION
收录类别	SCI
资助信息	National Natural Science Foundation of China [31000929]
语种	英语
WOS记录号	WOS:000333785900003
公开日期	2014-08-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20123]
专题	水生生物研究所_分析测试中心_期刊论文
作者单位	1.Huazhong Agr Univ, Key Lab Agromicrobial Resource & Dev, Minist Agr, Wuhan 430070, Hubei Province, Peoples R China 2.Huazhong Agr Univ, Inst Appl Mycol, Wuhan 430070, Hubei Province, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China 4.Guizhou Acad Agr Sci, Inst Crop Genet Resource, Guiyang 550006, Guizhou Provinc, Peoples R China 5.Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Gong, Wen-Bing,Liu, Wei,Lu, Ying-Ying,et al. Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes[J]. FUNGAL BIOLOGY,2014,118(3):295-308.
APA	Gong, Wen-Bing.,Liu, Wei.,Lu, Ying-Ying.,Bian, Yin-Bing.,Zhou, Yan.,...&Xiao, Yang.(2014).Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes.FUNGAL BIOLOGY,118(3),295-308.
MLA	Gong, Wen-Bing,et al."Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes".FUNGAL BIOLOGY 118.3(2014):295-308.