C1q-like Factor, a Target of miR-430, Regulates Primordial Germ Cell Development in Early Embryos of Carassius auratus

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	C1q-like Factor, a Target of miR-430, Regulates Primordial Germ Cell Development in Early Embryos of Carassius auratus
	Mei, Jie 1; Yue, Hua-Mei 2; Li, Zhi 2; Chen, Bo 2; Zhong, Jian-Xiang 2; Dan, Cheng 2; Zhou, Li 2; Gui, Jian-Fang 1,2
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
	2014
卷号	10 期号:1 页码:15-24
关键词	C1q-like microRNA miR-430 knockdown primordial germ cell early embryogenesis
ISSN号	1449-2288
通讯作者	Mei, J (reprint author), Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China.
中文摘要	C1q-like is a significant maternal factor of TNF/C1q super-family, and the abundant protein has been observed in both mature eggs of Carassius auratus and Carassius auratus gibelio, but its biological function in early embryo development has remained unclear. In this study, we firstly revealed a high level of maternal C1q-like transcript existence only in mature eggs of Carassius auratus, whereas no any maternal C1q-like transcript was observed in that of Carassius auratus gibelio. During embryonic development, the C1q-like zygotic expression begins around cardiopalmus stage in embryos of both Carassius auratus and Carassius auratus gibelio. Then, we examined the biological role of C1q-like by morpholino-mediated knockdown in early embryo development. Knockdown of CaOC1q resulted in a significant reduction of primordial germ cells (PGCs) in Carassius auratus, as shown by whole mount in situ hybridization with vasa-specific RNA probe, fluorescence immunostaining of vasa protein, and GFP imaging of the GFP-nanos1-3'UTR mRNA reporter. In vitro and in vivo evidence indicated that a microRNA, miR-430 could repress the C1q-like expression and PGC development. These data suggest that C1q-like should be a direct target of miR-430 and play an essential role in PGC development of Carassius auratus.
英文摘要	C1q-like is a significant maternal factor of TNF/C1q super-family, and the abundant protein has been observed in both mature eggs of Carassius auratus and Carassius auratus gibelio, but its biological function in early embryo development has remained unclear. In this study, we firstly revealed a high level of maternal C1q-like transcript existence only in mature eggs of Carassius auratus, whereas no any maternal C1q-like transcript was observed in that of Carassius auratus gibelio. During embryonic development, the C1q-like zygotic expression begins around cardiopalmus stage in embryos of both Carassius auratus and Carassius auratus gibelio. Then, we examined the biological role of C1q-like by morpholino-mediated knockdown in early embryo development. Knockdown of CaOC1q resulted in a significant reduction of primordial germ cells (PGCs) in Carassius auratus, as shown by whole mount in situ hybridization with vasa-specific RNA probe, fluorescence immunostaining of vasa protein, and GFP imaging of the GFP-nanos1-3'UTR mRNA reporter. In vitro and in vivo evidence indicated that a microRNA, miR-430 could repress the C1q-like expression and PGC development. These data suggest that C1q-like should be a direct target of miR-430 and play an essential role in PGC development of Carassius auratus.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biology
研究领域[WOS]	Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
关键词[WOS]	C1Q FAMILY-MEMBER ; MESSENGER-RNAS ; GIBEL CARP ; ZEBRAFISH MIR-430 ; STEM-CELLS ; EXPRESSION ; IDENTIFICATION ; PROLIFERATION ; MIGRATION ; GUIDANCE
收录类别	SCI
资助信息	National Key Basic Research Program [2010CB126301]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; Innovation Project of Chinese Academy of Sciences [KSCX3-EW-N-04]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]; Fundamental Research Funds for the Central Universities [52204-12018, 2013PY068]; National Natural Science Foundation of China [31301931]
语种	英语
WOS记录号	WOS:000329338400002
公开日期	2014-08-08
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20056]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Mei, Jie,Yue, Hua-Mei,Li, Zhi,et al. C1q-like Factor, a Target of miR-430, Regulates Primordial Germ Cell Development in Early Embryos of Carassius auratus[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2014,10(1):15-24.
APA	Mei, Jie.,Yue, Hua-Mei.,Li, Zhi.,Chen, Bo.,Zhong, Jian-Xiang.,...&Gui, Jian-Fang.(2014).C1q-like Factor, a Target of miR-430, Regulates Primordial Germ Cell Development in Early Embryos of Carassius auratus.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,10(1),15-24.
MLA	Mei, Jie,et al."C1q-like Factor, a Target of miR-430, Regulates Primordial Germ Cell Development in Early Embryos of Carassius auratus".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 10.1(2014):15-24.