Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L.

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L.
	Cao, Mengxi 1,2; Chen, Ji 1; Peng, Wei 1,2; Wang, Yaping 1; Liao, Lanjie 1; Li, Yongming 1; Trudeau, Vance L.3; Zhu, Zuoyan 1; Hu, Wei 1
刊名	GENERAL AND COMPARATIVE ENDOCRINOLOGY
	2014
卷号	195 页码:47-57
关键词	Growth hormone Reproduction Interaction Luteinizing hormone GH-transgenic common carp
ISSN号	0016-6480
通讯作者	Hu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
中文摘要	To study the complex interaction between growth and reproduction we have established lines of transgenic common carp (Cyprinus carpio) carrying a grass carp (Ctenopharyngodon idellus) growth hormone (GH) transgene. The GH-transgenic fish showed delayed gonadal development compared with non-transgenic common carp. To gain a better understanding of the phenomenon, we studied body growth, gonad development, changes of reproduction related genes and hormones of GH-transgenic common carp for 2 years. Over-expression of GH elevated peripheral gh transcription, serum GH levels, and inhibited endogenous GH expression in the pituitary. Hormone analyses indicated that GH-transgenic common carp had reduced pituitary and serum level of luteinizing hormone (LH). Among the tested genes, pituitary Ihfl was inhibited in GH-transgenic fish. Further analyses in vitro showed that GH inhibited Itil) expression. Localization of ghr with LH indicates the possibility of direct regulation of GH on gonadotrophs. We also found that GH-transgenic common carp had reduced pituitary sensitivity to stimulation by co-treatments with a salmon gonadotropin-releasing hormone (GnRH) agonist and a dopamine antagonist. Together these results suggest that the main cause of delayed reproductive development in GH transgenic common carp is reduced LH production and release. (C) 2013 Elsevier Inc. All rights reserved.
英文摘要	To study the complex interaction between growth and reproduction we have established lines of transgenic common carp (Cyprinus carpio) carrying a grass carp (Ctenopharyngodon idellus) growth hormone (GH) transgene. The GH-transgenic fish showed delayed gonadal development compared with non-transgenic common carp. To gain a better understanding of the phenomenon, we studied body growth, gonad development, changes of reproduction related genes and hormones of GH-transgenic common carp for 2 years. Over-expression of GH elevated peripheral gh transcription, serum GH levels, and inhibited endogenous GH expression in the pituitary. Hormone analyses indicated that GH-transgenic common carp had reduced pituitary and serum level of luteinizing hormone (LH). Among the tested genes, pituitary Ihfl was inhibited in GH-transgenic fish. Further analyses in vitro showed that GH inhibited Itil) expression. Localization of ghr with LH indicates the possibility of direct regulation of GH on gonadotrophs. We also found that GH-transgenic common carp had reduced pituitary sensitivity to stimulation by co-treatments with a salmon gonadotropin-releasing hormone (GnRH) agonist and a dopamine antagonist. Together these results suggest that the main cause of delayed reproductive development in GH transgenic common carp is reduced LH production and release. (C) 2013 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Endocrinology & Metabolism
研究领域[WOS]	Endocrinology & Metabolism
关键词[WOS]	GONADOTROPIN-RELEASING-HORMONE ; TRANSGENIC COHO SALMON ; TILAPIA OREOCHROMIS-NILOTICUS ; SUBUNIT GENE-EXPRESSION ; GOLDFISH CARASSIUS-AURATUS ; MESSENGER-RNA ; ONCORHYNCHUS-KISUTCH ; ATLANTIC SALMON ; FEEDBACK LOOP ; JAPANESE EEL
收录类别	SCI
资助信息	863 High Technology Project [2011AA100404]; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; University of Ottawa International Research Acceleration Program
语种	英语
WOS记录号	WOS:000330257100006
公开日期	2014-08-08
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20055]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Ottawa, Ctr Adv Res Environm Genom, Dept Biol, Ottawa, ON K1N 6N5, Canada
推荐引用方式 GB/T 7714	Cao, Mengxi,Chen, Ji,Peng, Wei,et al. Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L.[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2014,195:47-57.
APA	Cao, Mengxi.,Chen, Ji.,Peng, Wei.,Wang, Yaping.,Liao, Lanjie.,...&Hu, Wei.(2014).Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L..GENERAL AND COMPARATIVE ENDOCRINOLOGY,195,47-57.
MLA	Cao, Mengxi,et al."Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L.".GENERAL AND COMPARATIVE ENDOCRINOLOGY 195(2014):47-57.