Melanoma differentiation-associated gene 5 in zebrafish provoking higher interferon-promoter activity through signalling enhancing of its shorter splicing variant

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	Melanoma differentiation-associated gene 5 in zebrafish provoking higher interferon-promoter activity through signalling enhancing of its shorter splicing variant
	Zou, Peng Fei 1,2,3; Chang, Ming Xian 1; Xue, Na Na 1; Liu, Xue Qin 4; Li, Jun Hua 1; Fu, Jian Ping 1; Chen, Shan Nan 1; Nie, Pin 1,3
刊名	IMMUNOLOGY
	2014-02-01
卷号	141 期号:2 页码:192-202
关键词	melanoma differentiation-associated gene 5 mitochondrial antiviral signalling protein splicing variant type I interferon zebrafish
ISSN号	0019-2805
通讯作者	Chang, MX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China.
中文摘要	Melanoma differentiation-associated gene 5 (MDA5) is one of the three members in the retinoic acid-inducible gene I-like receptor (RLR) family, which are cytoplasmic pathogen recognition receptors recognizing intracellular viruses. In the present study, MDA5 and its spliced shorter forms, named as MDA5a and MDA5b, were identified in zebrafish. MDA5a and MDA5b can be up-regulated in cell lines following the infection of a negative ssRNA virus, the spring viraemia of carp virus (SVCV), and an intracellular Gram-negative bacterial pathogen Edwardsiella tarda, implying that the RLR may also be able to sense elements released from bacteria. The over-expression of MDA5a and MDA5b in fish cells resulted in significant induction of type I interferon promoter activity and enabled the protection of transfected cells against SVCV infection. Furthermore, the shorter spliced form, MDA5b when co-transfected with MDA5a or mitochondrial antiviral signalling protein (MAVS), induced a significantly higher level of interferon promoter activity, indicating that MDA5b may function as an enhancer in the interaction between MDA5 and MAVS.
英文摘要	Melanoma differentiation-associated gene 5 (MDA5) is one of the three members in the retinoic acid-inducible gene I-like receptor (RLR) family, which are cytoplasmic pathogen recognition receptors recognizing intracellular viruses. In the present study, MDA5 and its spliced shorter forms, named as MDA5a and MDA5b, were identified in zebrafish. MDA5a and MDA5b can be up-regulated in cell lines following the infection of a negative ssRNA virus, the spring viraemia of carp virus (SVCV), and an intracellular Gram-negative bacterial pathogen Edwardsiella tarda, implying that the RLR may also be able to sense elements released from bacteria. The over-expression of MDA5a and MDA5b in fish cells resulted in significant induction of type I interferon promoter activity and enabled the protection of transfected cells against SVCV infection. Furthermore, the shorter spliced form, MDA5b when co-transfected with MDA5a or mitochondrial antiviral signalling protein (MAVS), induced a significantly higher level of interferon promoter activity, indicating that MDA5b may function as an enhancer in the interaction between MDA5 and MAVS.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Immunology
研究领域[WOS]	Immunology
关键词[WOS]	I-LIKE RECEPTORS ; RIG-I ; EDWARDSIELLA-TARDA ; RNA HELICASE ; PATHOGEN RECOGNITION ; INNATE IMMUNITY ; MDA5 ; VIRUS ; ACTIVATION ; EXPRESSION
收录类别	SCI
资助信息	Basic Research Programme of China (973 Program) [2009CB118703]; National Natural Science Foundation of China [30830083]
语种	英语
WOS记录号	WOS:000329459000007
公开日期	2014-08-08
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20052]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei Province, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China 4.Huazhong Agr Univ, Coll Fisheries, Wuhan, Hubei Province, Peoples R China
推荐引用方式 GB/T 7714	Zou, Peng Fei,Chang, Ming Xian,Xue, Na Na,et al. Melanoma differentiation-associated gene 5 in zebrafish provoking higher interferon-promoter activity through signalling enhancing of its shorter splicing variant[J]. IMMUNOLOGY,2014,141(2):192-202.
APA	Zou, Peng Fei.,Chang, Ming Xian.,Xue, Na Na.,Liu, Xue Qin.,Li, Jun Hua.,...&Nie, Pin.(2014).Melanoma differentiation-associated gene 5 in zebrafish provoking higher interferon-promoter activity through signalling enhancing of its shorter splicing variant.IMMUNOLOGY,141(2),192-202.
MLA	Zou, Peng Fei,et al."Melanoma differentiation-associated gene 5 in zebrafish provoking higher interferon-promoter activity through signalling enhancing of its shorter splicing variant".IMMUNOLOGY 141.2(2014):192-202.