Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication

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	Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication
	Fu, Jian Ping 1,2; Luo, Zhang 1; Yan, Yang 3; Zou, Peng Fei 1; Zhang, Shu Huan 1; Qin, Qi Wei 3; Nie, Pin 1,4
刊名	FISH PATHOLOGY
	2013-12-01
卷号	48 期号:4 页码:126-134
关键词	cell line Pelodiscus sinensis Chinese soft-shelled turtle transfection soft-shelled turtle iridovirus STIV
ISSN号	0388-788X
通讯作者	Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
中文摘要	continuous cell line derived from extracranial carotid artery of the Chinese soft-shelled turtle Pelodiscus sinensis was established and characterized. It was named as STA (soft-shelled turtle artery) cell line. The cells were muscle-cell like, and were stained with antibody against smooth muscle myofilament marker, a-smooth muscle actin. These cells had a normal diploid chromosome number of 2n = 66, and their 12S rRNA and 16S rRNA sequences shared 99% identity with ones of the turtle in database. The cell line can be cultured well in media DMEM/F12 or M199 supplemented with 10% FBS at 28 C. It was further demonstrated that the cells were transfected successfully with pTurbo plasmid, with a transfection efficiency being over 30%. The soft-shelled turtle iridovirus (STIV) propagated in the cell line, causing typical CPE with the formation of inclusion bodies. It is suggested that the established STA cell line provides a convenient platform for studying the pathogenesis of STIV and biological aspects of the turtle.
英文摘要	continuous cell line derived from extracranial carotid artery of the Chinese soft-shelled turtle Pelodiscus sinensis was established and characterized. It was named as STA (soft-shelled turtle artery) cell line. The cells were muscle-cell like, and were stained with antibody against smooth muscle myofilament marker, a-smooth muscle actin. These cells had a normal diploid chromosome number of 2n = 66, and their 12S rRNA and 16S rRNA sequences shared 99% identity with ones of the turtle in database. The cell line can be cultured well in media DMEM/F12 or M199 supplemented with 10% FBS at 28 C. It was further demonstrated that the cells were transfected successfully with pTurbo plasmid, with a transfection efficiency being over 30%. The soft-shelled turtle iridovirus (STIV) propagated in the cell line, causing typical CPE with the formation of inclusion bodies. It is suggested that the established STA cell line provides a convenient platform for studying the pathogenesis of STIV and biological aspects of the turtle.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Veterinary Sciences
关键词[WOS]	SMOOTH-MUSCLE-CELLS ; GREEN SEA-TURTLE ; CHELONIA-MYDAS ; BODY PLAN ; EXPRESSION ; EVOLUTION ; DISEASE ; COMPLEX ; CULTURE
收录类别	SCI
资助信息	National Natural Science Foundation of China [31230075]; National Basic Research of Program (973 Program) [2009CB118703]
语种	英语
WOS记录号	WOS:000329960200003
公开日期	2014-08-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19973]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 4.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
推荐引用方式 GB/T 7714	Fu, Jian Ping,Luo, Zhang,Yan, Yang,et al. Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication[J]. FISH PATHOLOGY,2013,48(4):126-134.
APA	Fu, Jian Ping.,Luo, Zhang.,Yan, Yang.,Zou, Peng Fei.,Zhang, Shu Huan.,...&Nie, Pin.(2013).Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication.FISH PATHOLOGY,48(4),126-134.
MLA	Fu, Jian Ping,et al."Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication".FISH PATHOLOGY 48.4(2013):126-134.