The influence of microgravity on Euglena gracilis as studied on Shenzhou 8

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	The influence of microgravity on Euglena gracilis as studied on Shenzhou 8
	Nasir, A.1; Strauch, S. M.1; Becker, I.1; Sperling, A.1; Schuster, M.1; Richter, P. R.1; Weisskopf, M.1; Ntefidou, M.1; Daiker, V.1; An, Y. A.2
刊名	PLANT BIOLOGY
	2014
卷号	16 页码:113-119
关键词	Euglena gracilis gene expression microgravity qPCR space flight unicellular flagellate
ISSN号	1435-8603
通讯作者	Richter, PR (reprint author), Univ Erlangen Nurnberg, Dept Biol, Dept Cell Biol, D-91054 Erlangen, Germany.
中文摘要	The German Aerospace Center (DLR) enabled German participation in the joint space campaign on the unmanned Shenzhou 8 spacecraft in November 2011. In this report, the effect of microgravity on Euglena gracilis cells is described. Custom-made dual compartment cell fixation units (containing cells in one chamber and fixative - RNA lysis buffer - in another one) were enclosed in a small container and placed in the Simbox incubator, which is an experiment support system. Cells were fixed by injecting them with fixative at different time intervals. In addition to stationary experiment slots, Simbox provides a 1g reference centrifuge. Cell fixation units were mounted in microgravity and 1g reference positions of Simbox. Two Simbox incubators were used, one for space flight and the other as ground reference. Cells were fixed soon after launch and shortly before return of the spaceship. Due to technical problems, only early in-flight samples (about 40min after launch microgravity and corresponding 1g reference) were fully mixed with fixative, therefore only data from those samples are presented. Transcription of several genes involved in signal transduction, oxidative stress defence, cell cycle regulation and heat shock responses was investigated with quantitative PCR. The data indicate that Euglena cells suffer stress upon short-term exposure to microgravity; various stress-induced genes were up-regulated. Of 32 tested genes, 18 were up-regulated, one down-regulated and the rest remained unaltered. These findings are in a good agreement with results from other research groups using other organisms.
英文摘要	The German Aerospace Center (DLR) enabled German participation in the joint space campaign on the unmanned Shenzhou 8 spacecraft in November 2011. In this report, the effect of microgravity on Euglena gracilis cells is described. Custom-made dual compartment cell fixation units (containing cells in one chamber and fixative - RNA lysis buffer - in another one) were enclosed in a small container and placed in the Simbox incubator, which is an experiment support system. Cells were fixed by injecting them with fixative at different time intervals. In addition to stationary experiment slots, Simbox provides a 1g reference centrifuge. Cell fixation units were mounted in microgravity and 1g reference positions of Simbox. Two Simbox incubators were used, one for space flight and the other as ground reference. Cells were fixed soon after launch and shortly before return of the spaceship. Due to technical problems, only early in-flight samples (about 40min after launch microgravity and corresponding 1g reference) were fully mixed with fixative, therefore only data from those samples are presented. Transcription of several genes involved in signal transduction, oxidative stress defence, cell cycle regulation and heat shock responses was investigated with quantitative PCR. The data indicate that Euglena cells suffer stress upon short-term exposure to microgravity; various stress-induced genes were up-regulated. Of 32 tested genes, 18 were up-regulated, one down-regulated and the rest remained unaltered. These findings are in a good agreement with results from other research groups using other organisms.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	RANDOM POSITIONING MACHINE ; GENE-EXPRESSION PATTERNS ; SIMULATED MICROGRAVITY ; ARABIDOPSIS-THALIANA ; INCREASES APOPTOSIS ; SIGNAL-TRANSDUCTION ; ALTERED GRAVITY ; FLAGELLATE ; CELLS ; CYTOSKELETON
收录类别	SCI
资助信息	DLR on behalf of the BMBF [50WB1128]
语种	英语
WOS记录号	WOS:000328827200016
公开日期	2014-08-08
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20074]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	1.Univ Erlangen Nurnberg, Dept Biol, Div Cell Biol, D-91054 Erlangen, Germany 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Nasir, A.,Strauch, S. M.,Becker, I.,et al. The influence of microgravity on Euglena gracilis as studied on Shenzhou 8[J]. PLANT BIOLOGY,2014,16:113-119.
APA	Nasir, A..,Strauch, S. M..,Becker, I..,Sperling, A..,Schuster, M..,...&Lebert, M..(2014).The influence of microgravity on Euglena gracilis as studied on Shenzhou 8.PLANT BIOLOGY,16,113-119.
MLA	Nasir, A.,et al."The influence of microgravity on Euglena gracilis as studied on Shenzhou 8".PLANT BIOLOGY 16(2014):113-119.