Drosophila Pheromone-Sensing Neurons Expressing the ppk25 Ion Channel Subunit Stimulate Male Courtship and Female Receptivity

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	Drosophila Pheromone-Sensing Neurons Expressing the ppk25 Ion Channel Subunit Stimulate Male Courtship and Female Receptivity
	Vijayan, V ; Thistle, R ; Liu, T ; Starostina, E ; Pikielny, CW
刊名	PLOS GENETICS
	2014
卷号	10 期号:3 页码:547-561
关键词	BEHAVIORAL-RESPONSES TARGETED EXPRESSION OLFACTORY CIRCUIT GUSTATORY NEURONS GENE-EXPRESSION SEX-PHEROMONE MELANOGASTER DEG/ENAC RECEPTOR MUTANT
ISSN号	1553-7390
通讯作者	Vijayan, V (reprint author), Geisel Sch Med Dartmouth, Dept Genet, Hanover, NH 03755 USA.,claudio.pikielny@dartmouth.edu
英文摘要	As in many species, gustatory pheromones regulate the mating behavior of Drosophila. Recently, several ppk genes, encoding ion channel subunits of the DEG/ENaC family, have been implicated in this process, leading to the identification of gustatory neurons that detect specific pheromones. In a subset of taste hairs on the legs of Drosophila, there are two ppk23-expressing, pheromone-sensing neurons with complementary response profiles; one neuron detects female pheromones that stimulate male courtship, the other detects male pheromones that inhibit male-male courtship. In contrast to ppk23, ppk25, is only expressed in a single gustatory neuron per taste hair, and males with impaired ppk25 function court females at reduced rates but do not display abnormal courtship of other males. These findings raised the possibility that ppk25 expression defines a subset of pheromone-sensing neurons. Here we show that ppk25 is expressed and functions in neurons that detect female-specific pheromones and mediates their stimulatory effect on male courtship. Furthermore, the role of ppk25 and ppk25-expressing neurons is not restricted to responses to female-specific pheromones. ppk25 is also required in the same subset of neurons for stimulation of male courtship by young males, males of the Tai2 strain, and by synthetic 7-pentacosene (7-P), a hydrocarbon normally found at low levels in both males and females. Finally, we unexpectedly find that, in females, ppk25 and ppk25-expressing cells regulate receptivity to mating. In the absence of the third antennal segment, which has both olfactory and auditory functions, mutations in ppk25 or silencing of ppk25-expressing neurons block female receptivity to males. Together these results indicate that ppk25 identifies a functionally specialized subset of pheromone-sensing neurons. While ppk25 neurons are required for the responses to multiple pheromones, in both males and females these neurons are specifically involved in stimulating courtship and mating.
学科主题	Genetics & Heredity
收录类别	SCI
资助信息	NIDCD [R01DC007911]; Howard Hughes Medical Institute [76200-560801]; NSF
语种	英语
公开日期	2014-07-30
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/2619]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Vijayan, V,Thistle, R,Liu, T,et al. Drosophila Pheromone-Sensing Neurons Expressing the ppk25 Ion Channel Subunit Stimulate Male Courtship and Female Receptivity[J]. PLOS GENETICS,2014,10(3):547-561.
APA	Vijayan, V,Thistle, R,Liu, T,Starostina, E,&Pikielny, CW.(2014).Drosophila Pheromone-Sensing Neurons Expressing the ppk25 Ion Channel Subunit Stimulate Male Courtship and Female Receptivity.PLOS GENETICS,10(3),547-561.
MLA	Vijayan, V,et al."Drosophila Pheromone-Sensing Neurons Expressing the ppk25 Ion Channel Subunit Stimulate Male Courtship and Female Receptivity".PLOS GENETICS 10.3(2014):547-561.