Molecular Cloning, Characterization, and Expression of Two Myeloid Differentiation Factor 88 (Myd88) in Pacific Oyster, Crassostrea gigas

	Molecular Cloning, Characterization, and Expression of Two Myeloid Differentiation Factor 88 (Myd88) in Pacific Oyster, Crassostrea gigas
	Du, Yishuai 1,2; Zhang, Linlin 1; Huang, Baoyu1,2 ; Guan, Xudong1,2 ; Li, Li 1; Zhang, Guofan 1; Li, L
刊名	JOURNAL OF THE WORLD AQUACULTURE SOCIETY
	2013-12-01
卷号	44 期号:6 页码:759-774
ISSN号	0893-8849
通讯作者	Li, L
中文摘要	Myeloid differentiation factor 88 (MyD88) is a universal adaptor protein and plays an important role in the signal transduction of Toll/interleukin-1 receptor (TIR/IL-1R) family. In this study, two novel molluskan MyD88 family members (named as CgMyD88-1 and CgMyD88-2) were identified from the Pacific oyster, Crassostrea gigas. The full-length cDNA of CgMyD88-1 was of 2453 base pairs (bp), with an open reading frame (ORF) of 1077bp encoding 358 amino acids. The cDNA of CgMyD88-2 consists of 1374bp ORF encoding 457 amino acids. Both CgMyD88s contain death domain (DD) and TIR domain which are typical features of MyD88 family proteins. Three conserved boxes were also found in the two CgMyD88s which were similar to MyD88s in vertebrates. The expression level of both CgMyD88 mRNAs in selected tissues was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Both CgMyD88 transcripts were constitutively expressed in all tested tissues. Gill and hemocytes were shown with highest expression levels while adductor muscle and gonad were with lower levels. The CgMyD88 transcripts in hemocytes after ostreid herpesvirus 1 (OsHV-1) challenge were also determined by qRT-PCR. Both CgMyD88s were significantly upregulated in hemocytes after OsHV-1 challenge and had significant differences (P < 0.05) at some time points compared with the control. Our results as previously reported suggest that MyD88-dependent pathway exists in C. gigas, and its critical adaptor CgMyD88 plays important roles in oysters' innate immune defense against OsHV-1 infection.
英文摘要	Myeloid differentiation factor 88 (MyD88) is a universal adaptor protein and plays an important role in the signal transduction of Toll/interleukin-1 receptor (TIR/IL-1R) family. In this study, two novel molluskan MyD88 family members (named as CgMyD88-1 and CgMyD88-2) were identified from the Pacific oyster, Crassostrea gigas. The full-length cDNA of CgMyD88-1 was of 2453 base pairs (bp), with an open reading frame (ORF) of 1077bp encoding 358 amino acids. The cDNA of CgMyD88-2 consists of 1374bp ORF encoding 457 amino acids. Both CgMyD88s contain death domain (DD) and TIR domain which are typical features of MyD88 family proteins. Three conserved boxes were also found in the two CgMyD88s which were similar to MyD88s in vertebrates. The expression level of both CgMyD88 mRNAs in selected tissues was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Both CgMyD88 transcripts were constitutively expressed in all tested tissues. Gill and hemocytes were shown with highest expression levels while adductor muscle and gonad were with lower levels. The CgMyD88 transcripts in hemocytes after ostreid herpesvirus 1 (OsHV-1) challenge were also determined by qRT-PCR. Both CgMyD88s were significantly upregulated in hemocytes after OsHV-1 challenge and had significant differences (P < 0.05) at some time points compared with the control. Our results as previously reported suggest that MyD88-dependent pathway exists in C. gigas, and its critical adaptor CgMyD88 plays important roles in oysters' innate immune defense against OsHV-1 infection.
学科主题	Fisheries
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries
研究领域[WOS]	Fisheries
关键词[WOS]	REAL-TIME PCR ; TOLL-LIKE RECEPTORS ; I-KAPPA-B ; OSTREID HERPESVIRUS-1 ; STRUCTURAL BASIS ; ADAPTER PROTEIN ; TIR DOMAIN ; FAMILY ; VIRUS ; INFECTION
收录类别	SCI
原文出处	10.1111/jwas.12077
语种	英语
WOS记录号	WOS:000327822400001
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16708]
专题	海洋研究所_海洋生物技术研发中心
通讯作者	Li, L
作者单位	1.Chinese Acad Sci, Inst Oceanol, Lab Marine Molluscan Genet & Breeding, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
推荐引用方式 GB/T 7714	Du, Yishuai,Zhang, Linlin,Huang, Baoyu,et al. Molecular Cloning, Characterization, and Expression of Two Myeloid Differentiation Factor 88 (Myd88) in Pacific Oyster, Crassostrea gigas[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2013,44(6):759-774.
APA	Du, Yishuai.,Zhang, Linlin.,Huang, Baoyu.,Guan, Xudong.,Li, Li.,...&Li, L.(2013).Molecular Cloning, Characterization, and Expression of Two Myeloid Differentiation Factor 88 (Myd88) in Pacific Oyster, Crassostrea gigas.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,44(6),759-774.
MLA	Du, Yishuai,et al."Molecular Cloning, Characterization, and Expression of Two Myeloid Differentiation Factor 88 (Myd88) in Pacific Oyster, Crassostrea gigas".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 44.6(2013):759-774.