Low Molecular Weight Fucoidan against Renal Ischemia-Reperfusion Injury via Inhibition of the MAPK Signaling Pathway

	Low Molecular Weight Fucoidan against Renal Ischemia-Reperfusion Injury via Inhibition of the MAPK Signaling Pathway
	Chen, Jihui 1,2; Wang, Weiling 1,2; Zhang, Quanbin 3; Li, Fei 1,2; Lei, Tianluo 1,2; Luo, Dali 4; Zhou, Hong 1,2; Yang, Baoxue 1,2
刊名	PLOS ONE
	2013-02-13
卷号	8 期号:2 页码:e562240
ISSN号	1932-6203
通讯作者	Zhou, H
中文摘要	Background: Ischemia reperfusion injury (IRI) is a leading cause of acute kidney injury (AKI) in both native and transplanted kidneys. The objective of the present study was to evaluate whether low-molecular-weight fucoidan (LMWF) could attenuate renal IRI in an animal model and in vitro cell models and study the mechanisms in which LMWF protected from IRI. Methodology/Principal Findings: Male mice were subjected to right renal ischemia for 30 min and reperfusion for 24 h, or to a sham operation with left kidney removed. Kidneys undergone IR showed characteristic morphological changes, such as tubular dilatation, and brush border loss. However, LMWF significantly corrected the renal dysfunction and the abnormal levels of MPO, MDA and SOD induced by IR. LMWF also inhibited the activation of MAPK pathways, which consequently resulted in a significant decrease in the release of cytochrome c from mitochondria, ratios of Bax/Bcl-2 and cleaved caspase3/ caspase-3, and phosphorylation of p53. LMWF alleviated hypoxia-reoxygenation or CoCl2 induced cell viability loss and Delta Psi m dissipation in HK2 renal tubular epithelial cells, which indicates LMWF may result in an inhibition of the apoptosis pathway through reducing activity of MAPK pathways in a dose-dependent manner. Conclusions/Significance: Our in vivo and in vitro studies show that LMWF ameliorates acute renal IRI via inhibiting MAPK signaling pathways. The data provide evidence that LMWF may serve as a potential therapeutic agent for acute renal IRI.
英文摘要	Background: Ischemia reperfusion injury (IRI) is a leading cause of acute kidney injury (AKI) in both native and transplanted kidneys. The objective of the present study was to evaluate whether low-molecular-weight fucoidan (LMWF) could attenuate renal IRI in an animal model and in vitro cell models and study the mechanisms in which LMWF protected from IRI.
学科主题	Science & Technology - Other Topics
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	UNDARIA-PINNATIFIDA ; MYOCARDIAL-ISCHEMIA ; PC12 CELLS ; LAMINARIA-JAPONICA ; FREE-RADICALS ; IN-VIVO ; APOPTOSIS ; ACTIVATION ; RAT ; JNK
收录类别	SCI
原文出处	10.1371/journal.pone.0056224
语种	英语
WOS记录号	WOS:000315970300131
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16707]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100871, Peoples R China 2.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 4.Capital Med Univ, Dept Pharmacol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jihui,Wang, Weiling,Zhang, Quanbin,et al. Low Molecular Weight Fucoidan against Renal Ischemia-Reperfusion Injury via Inhibition of the MAPK Signaling Pathway[J]. PLOS ONE,2013,8(2):e562240.
APA	Chen, Jihui.,Wang, Weiling.,Zhang, Quanbin.,Li, Fei.,Lei, Tianluo.,...&Yang, Baoxue.(2013).Low Molecular Weight Fucoidan against Renal Ischemia-Reperfusion Injury via Inhibition of the MAPK Signaling Pathway.PLOS ONE,8(2),e562240.
MLA	Chen, Jihui,et al."Low Molecular Weight Fucoidan against Renal Ischemia-Reperfusion Injury via Inhibition of the MAPK Signaling Pathway".PLOS ONE 8.2(2013):e562240.