Extraction and Separation of Fucoidan from Laminaria japonica with Chitosan as Extractant

	Extraction and Separation of Fucoidan from Laminaria japonica with Chitosan as Extractant
	Xing, Ronge; Liu, Song; Yu, Huahua; Chen, Xiaolin; Qin, Yukun; Li, Kecheng; Li, Pengcheng; Li, PC
刊名	BIOMED RESEARCH INTERNATIONAL
	2013
页码	193689
ISSN号	2314-6133
通讯作者	Li, PC
中文摘要	Herein the extraction method of fucoidan from Laminaria japonica is reported. Firstly, chitosan, chitosan-N-2-hydroxypropyl trimethyl ammonium chloride (HACC), and hexadecyltrimethylammonium bromide (CPAB) were used to extract the fucoidan. The results showed that chitosan was the optimal extractant compared with the other two extractants. After extraction, different aqueous solutions, including NaCl, KCl, and HCl (pH2), were used to separate fucoidan from chitosan-fucoidan complex. The results showed that the separation ability of NaCl was slightly higher than that of KCl. Moreover, the price of NaCl is lower than that of KCl. Given the quality-price rate, NaCl solution was chosen as the separation solution. Thirdly, the concentration and ratio of NaCl solution : sediment influence the separation of fucoidan from chitosan-fucoidan complex. The results showed that the optimal separation conditions include 4mol/L NaCl solution with the ratio of NaCl solution to sediment at 30:1. Fucoidan content was found to be affected by different separation time. Fucoidan content increased with the increase of separation time, and the optimal separation time was 6 h. Compared with traditional alkali extraction method, this method not only reduces the usage of alkali and acid and alleviate environment pollution, but also has the comparable extraction yield of fucoidan. It is a potential method for extraction of fucoidan.
英文摘要	Herein the extraction method of fucoidan from Laminaria japonica is reported. Firstly, chitosan, chitosan-N-2-hydroxypropyl trimethyl ammonium chloride (HACC), and hexadecyltrimethylammonium bromide (CPAB) were used to extract the fucoidan. The results showed that chitosan was the optimal extractant compared with the other two extractants. After extraction, different aqueous solutions, including NaCl, KCl, and HCl (pH2), were used to separate fucoidan from chitosan-fucoidan complex. The results showed that the separation ability of NaCl was slightly higher than that of KCl. Moreover, the price of NaCl is lower than that of KCl. Given the quality-price rate, NaCl solution was chosen as the separation solution. Thirdly, the concentration and ratio of NaCl solution : sediment influence the separation of fucoidan from chitosan-fucoidan complex. The results showed that the optimal separation conditions include 4mol/L NaCl solution with the ratio of NaCl solution to sediment at 30:1. Fucoidan content was found to be affected by different separation time. Fucoidan content increased with the increase of separation time, and the optimal separation time was 6 h. Compared with traditional alkali extraction method, this method not only reduces the usage of alkali and acid and alleviate environment pollution, but also has the comparable extraction yield of fucoidan. It is a potential method for extraction of fucoidan.
学科主题	Biotechnology & Applied Microbiology ; Research & Experimental Medicine
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
研究领域[WOS]	Biotechnology & Applied Microbiology ; Research & Experimental Medicine
关键词[WOS]	BROWN SEAWEED ; POLYSACCHARIDES ; NMR
收录类别	SCI
原文出处	10.1155/2013/193689
语种	英语
WOS记录号	WOS:000327952600001
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16698]
专题	海洋研究所_海洋生物技术研发中心海洋研究所_海洋环境工程技术研究发展中心
通讯作者	Li, PC
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Xing, Ronge,Liu, Song,Yu, Huahua,et al. Extraction and Separation of Fucoidan from Laminaria japonica with Chitosan as Extractant[J]. BIOMED RESEARCH INTERNATIONAL,2013:193689.
APA	Xing, Ronge.,Liu, Song.,Yu, Huahua.,Chen, Xiaolin.,Qin, Yukun.,...&Li, PC.(2013).Extraction and Separation of Fucoidan from Laminaria japonica with Chitosan as Extractant.BIOMED RESEARCH INTERNATIONAL,193689.
MLA	Xing, Ronge,et al."Extraction and Separation of Fucoidan from Laminaria japonica with Chitosan as Extractant".BIOMED RESEARCH INTERNATIONAL (2013):193689.