Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus
	Sun, L. N.1,2; Yang, H. S.1; Chen, M. Y.3; Xu, D. X.1,2; Yang, HS
刊名	GENETICS AND MOLECULAR RESEARCH
	2013
卷号	12 期号:4 页码:5321-5334
关键词	Wnt6 Hox6 Sea cucumber Intestinal regeneration
ISSN号	1676-5680
通讯作者	Yang, HS
中文摘要	Many developmental genes play important roles in regeneration. Here, we cloned the two developmental genes, Wnt6 and Hox6, from Apostichopus japonicus by rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR). The Wnt6 full-length cDNA is 1140 bp long with a 1029-bp open reading frame encoding 342 amino acids. The deduced Wnt6 protein contained a 22-amino acid signal peptide and a 274-amino acid mature peptide. The Hox6 (AjHBOX6) full-length cDNA contained 1312 bp with a 966-bp open reading frame encoding 321 amino acids. Its deduced amino acid sequence included one homeodomain (60 amino acids) and three domains with low compositional complexity. Comparison and phylogenetic analysis revealed that Wnt6 and Hox6 in A. japonicus had the nearest relationship to those in sea urchins. Further expression analysis by real-time PCR showed that Wnt6 and Hox6 were apparently upregulated during intestine regeneration, which suggested that they were involved in the intestinal regeneration of sea cucumbers.
英文摘要	Many developmental genes play important roles in regeneration. Here, we cloned the two developmental genes, Wnt6 and Hox6, from Apostichopus japonicus by rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR). The Wnt6 full-length cDNA is 1140 bp long with a 1029-bp open reading frame encoding 342 amino acids. The deduced Wnt6 protein contained a 22-amino acid signal peptide and a 274-amino acid mature peptide. The Hox6 (AjHBOX6) full-length cDNA contained 1312 bp with a 966-bp open reading frame encoding 321 amino acids. Its deduced amino acid sequence included one homeodomain (60 amino acids) and three domains with low compositional complexity. Comparison and phylogenetic analysis revealed that Wnt6 and Hox6 in A. japonicus had the nearest relationship to those in sea urchins. Further expression analysis by real-time PCR showed that Wnt6 and Hox6 were apparently upregulated during intestine regeneration, which suggested that they were involved in the intestinal regeneration of sea cucumbers.
学科主题	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Genetics & Heredity
研究领域[WOS]	Biochemistry & Molecular Biology ; Genetics & Heredity
关键词[WOS]	GLABERRIMA SELENKA HOLOTHUROIDEA ; VISCERAL REGENERATION ; LIMB MYOGENESIS ; AXOLOTL LIMBS ; GENE ; MECHANISMS ; ROLES ; ECHINODERMATA ; SEQUENCES ; EPENDYMIN
收录类别	SCI
原文出处	10.4238/2013.November.7.7
语种	英语
WOS记录号	WOS:000331608000121
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16465]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Yang, HS
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Ocean Univ China, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Sun, L. N.,Yang, H. S.,Chen, M. Y.,et al. Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus[J]. GENETICS AND MOLECULAR RESEARCH,2013,12(4):5321-5334.
APA	Sun, L. N.,Yang, H. S.,Chen, M. Y.,Xu, D. X.,&Yang, HS.(2013).Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus.GENETICS AND MOLECULAR RESEARCH,12(4),5321-5334.
MLA	Sun, L. N.,et al."Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus".GENETICS AND MOLECULAR RESEARCH 12.4(2013):5321-5334.