The increased transcriptional response and translocation of a Rel/NF-kappa B homologue in scallop Chlamys farreri during the immune stimulation

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	The increased transcriptional response and translocation of a Rel/NF-kappa B homologue in scallop Chlamys farreri during the immune stimulation
	Zhou, Zhi 1; Wang, Mengqiang1 ; Zhao, Jianmin 1; Wang, Lingling 1; Gao, Yang1,2 ; Zhang, Huan 1; Liu, Rui 1; Song, Linsheng 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2013-05-01
卷号	34 期号:5 页码:1209-1215
关键词	Rel NF-kappa B Transcription activity Immunomodulation Scallop
ISSN号	1050-4648
通讯作者	Wang, LL
中文摘要	The Rel/NF-kappa B transcription factors can function as key regulators to modulate the expression of immune-related genes in response to immune challenge or environmental stress. In the present study, a gene coding Rel/NF-kappa B homologue was identified from scallop Chlamys farreri (designated CfRel). Its deduced protein comprised 359 amino acids, and contained a conserved N-terminal Rel homology domain (RHO) and an IPT domain. There was an NF-kappa B/Rel/dorsal domain signature sequence in the RHD domain. The mRNA transcripts of CfRel could be detected in all the tested tissues including adductor muscle, mantle, gill, gonad, haemocytes, kidney and hepatopancreas, with the highest expression level in hepatopancreas. After LPS stimulation, there were two peaks of CfRel mRNA expression level in haemocytes at 6 h (25.25-fold, P < 0.05) and 24 h (59.66-fold, P < 0.05) respectively, while the mRNA expression of CfRel was only up-regulated at 3 h after PGN stimulation (2.35-fold, P < 0.05). By Western blotting technique, CfRel protein was observed in the cytoplasm and nucleus of scallop haemocytes, and its concentration in the haemocyte nucleus increased significantly at 3 h and 12 h after LPS stimulation. The noticeable NF-kappa B transcription activity of CfRel protein was determined by NF-kappa B luciferase reporter assays (122.43%, P < 0.05), and it decreased significantly (17.61%, P < 0.05) after the coexpression of scallop I kappa B protein. These results collectively suggested that CfRel mRNA transcripts and protein were induced by immune stimulation, and CfRel protein could extricate itself from I kappa B protein and transfer into the haemocyte nucleus to modulate the immune response in scallop. (C) 2013 Elsevier Ltd. All rights reserved.
英文摘要	The Rel/NF-kappa B transcription factors can function as key regulators to modulate the expression of immune-related genes in response to immune challenge or environmental stress. In the present study, a gene coding Rel/NF-kappa B homologue was identified from scallop Chlamys farreri (designated CfRel). Its deduced protein comprised 359 amino acids, and contained a conserved N-terminal Rel homology domain (RHO) and an IPT domain. There was an NF-kappa B/Rel/dorsal domain signature sequence in the RHD domain. The mRNA transcripts of CfRel could be detected in all the tested tissues including adductor muscle, mantle, gill, gonad, haemocytes, kidney and hepatopancreas, with the highest expression level in hepatopancreas. After LPS stimulation, there were two peaks of CfRel mRNA expression level in haemocytes at 6 h (25.25-fold, P < 0.05) and 24 h (59.66-fold, P < 0.05) respectively, while the mRNA expression of CfRel was only up-regulated at 3 h after PGN stimulation (2.35-fold, P < 0.05). By Western blotting technique, CfRel protein was observed in the cytoplasm and nucleus of scallop haemocytes, and its concentration in the haemocyte nucleus increased significantly at 3 h and 12 h after LPS stimulation. The noticeable NF-kappa B transcription activity of CfRel protein was determined by NF-kappa B luciferase reporter assays (122.43%, P < 0.05), and it decreased significantly (17.61%, P < 0.05) after the coexpression of scallop I kappa B protein. These results collectively suggested that CfRel mRNA transcripts and protein were induced by immune stimulation, and CfRel protein could extricate itself from I kappa B protein and transfer into the haemocyte nucleus to modulate the immune response in scallop. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	C-TYPE LECTIN ; DROSOPHILA-MELANOGASTER ; SIGNALING PATHWAYS ; CRASSOSTREA-GIGAS ; PACIFIC OYSTER ; KINASE COMPLEX ; ACTIVATION ; HEMOCYTES ; PROTEIN ; EXPRESSION
收录类别	SCI
原文出处	10.1016/j.fsi.2013.01.009
语种	英语
WOS记录号	WOS:000317870600023
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16649]
专题	海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋腐蚀与防护研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Zhi,Wang, Mengqiang,Zhao, Jianmin,et al. The increased transcriptional response and translocation of a Rel/NF-kappa B homologue in scallop Chlamys farreri during the immune stimulation[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1209-1215.
APA	Zhou, Zhi.,Wang, Mengqiang.,Zhao, Jianmin.,Wang, Lingling.,Gao, Yang.,...&Song, Linsheng.(2013).The increased transcriptional response and translocation of a Rel/NF-kappa B homologue in scallop Chlamys farreri during the immune stimulation.FISH & SHELLFISH IMMUNOLOGY,34(5),1209-1215.
MLA	Zhou, Zhi,et al."The increased transcriptional response and translocation of a Rel/NF-kappa B homologue in scallop Chlamys farreri during the immune stimulation".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1209-1215.