Spermatozoid life-span of two brown seaweeds, Saccharina japonica and Undaria pinnatifida, as measured by fertilization efficiency

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	Spermatozoid life-span of two brown seaweeds, Saccharina japonica and Undaria pinnatifida, as measured by fertilization efficiency
	Li Jing1,2 ; Pang Shaojun 1; Liu Feng 1; Shan Tifeng 1; Gao Suqin 1
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2013-07-01
卷号	31 期号:4 页码:774-781
关键词	gametogenesis life-span of sperm Saccharina japonica seaweeds Undaria pinnatifida
ISSN号	0254-4059
通讯作者	Pang, SJ
中文摘要	During sexual reproduction of seaweeds, spermatozoid (sperm) discharge is triggered by chemical messengers (pheromones) released by the female gametes. The chemotactic ability of the sperm ensures fertilization success. Using unialgal male and female gametophyte material under designated standard gametogenesis testing (SGT) conditions, the potential life-span of the sperm of two seaweeds, Saccharina japonica and Undaria pinnatifida, was assessed by their ability to fertilize eggs. Results show that within 20-30 min after being discharged, sperm of both species could complete fertilization without an apparent decline in fertilization rate. Although fertilization rate 60-120 min after sperm discharge dropped significantly in both species, some sperm were viable enough to fertilize the eggs. In S. japonica, at 12A degrees C, some sperm were able to fertilize eggs up to 12 h after discharge. In both species, egg discharge rates (EDR) in the male and female mixed positive controls were significantly higher than those of all the sperm-testing groups. Doubling the seeded male gametophytes of S. japonica in the SGT tests significantly increased the EDR, further confirming the effect of the presence of the male on the female in terms of facilitating egg discharge from oogonia.
英文摘要	During sexual reproduction of seaweeds, spermatozoid (sperm) discharge is triggered by chemical messengers (pheromones) released by the female gametes. The chemotactic ability of the sperm ensures fertilization success. Using unialgal male and female gametophyte material under designated standard gametogenesis testing (SGT) conditions, the potential life-span of the sperm of two seaweeds, Saccharina japonica and Undaria pinnatifida, was assessed by their ability to fertilize eggs. Results show that within 20-30 min after being discharged, sperm of both species could complete fertilization without an apparent decline in fertilization rate. Although fertilization rate 60-120 min after sperm discharge dropped significantly in both species, some sperm were viable enough to fertilize the eggs. In S. japonica, at 12A degrees C, some sperm were able to fertilize eggs up to 12 h after discharge. In both species, egg discharge rates (EDR) in the male and female mixed positive controls were significantly higher than those of all the sperm-testing groups. Doubling the seeded male gametophytes of S. japonica in the SGT tests significantly increased the EDR, further confirming the effect of the presence of the male on the female in terms of facilitating egg discharge from oogonia.
学科主题	Marine & Freshwater Biology ; Oceanography
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Limnology ; Oceanography
研究领域[WOS]	Marine & Freshwater Biology ; Oceanography
关键词[WOS]	POLLEN ; PHAEOPHYCEAE ; POLLINATION ; LAMINARIALES ; SPOROPHYTES ; SEAGRASSES ; INDUCTION ; CULTURE ; RELEASE
收录类别	SCI
原文出处	10.1007/s00343-013-2207-y
语种	英语
WOS记录号	WOS:000321629400007
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16635]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li Jing,Pang Shaojun,Liu Feng,et al. Spermatozoid life-span of two brown seaweeds, Saccharina japonica and Undaria pinnatifida, as measured by fertilization efficiency[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(4):774-781.
APA	Li Jing,Pang Shaojun,Liu Feng,Shan Tifeng,&Gao Suqin.(2013).Spermatozoid life-span of two brown seaweeds, Saccharina japonica and Undaria pinnatifida, as measured by fertilization efficiency.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(4),774-781.
MLA	Li Jing,et al."Spermatozoid life-span of two brown seaweeds, Saccharina japonica and Undaria pinnatifida, as measured by fertilization efficiency".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.4(2013):774-781.