Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China
	Li, Jialin 1; Li, Fuchao 2; Yu, Shuxian 1,3; Qin, Song 1; Wang, Guangyi 4,5
刊名	MICROBIAL ECOLOGY
	2013-11-01
卷号	66 期号:4 页码:861-870
ISSN号	0095-3628
通讯作者	Qin, S
中文摘要	Mariculture is one of the major seafood supplies worldwide and has caused serious environmental concerns on the coastal zone. Its rapid development has been shown to disrupt the sediment ecosystems and thus influence the benthic bacterial communities. Bacterial diversity and community structure within both adjacent farms and non-cultured zones intertidal sediments along the coasts of Qinhuangdao and Dalian, China, were investigated using full-length 16S rRNA gene-based T-RFLP analyses and clone library construction. Richness and Shannon-Wiener index were significantly increased at sites adjacent the mariculture farm with mean values of 29 and 2.97 from peak profiles of T-RFLP result. Clustering analyses suggested that impacts of mariculture on bacterial diversity of sediment were significantly larger than those resulted from temporal and spatial scales. Upon comparisons of RFLP patterns from 602 clones from libraries of the selected five samples, 137 OTUs were retrieved. Members of gamma- and delta-Proteobacteria, Bacilli, Flavobacteria, and Actinobacteria were recorded in all libraries. In addition, gamma-Proteobacteria were dominant in all samples (21.7 similar to 45.0 %). Redundancy analysis revealed that the distribution of bacterial composition seemed to be determined by the variables of salinity, PO4 (3-)-P, NH4 (+)-N, and Chlorophyll a content. The phyla of gamma-Proteobacteria, Clostridia, Flavobacteria, Bacilli, and Planctomycetes were principal components to contribute to the bacterial differences of clone libraries. Our finding demonstrated that these phyla could display variations of bacterial composition linked to environmental disturbance resulted from mariculture.
英文摘要	Mariculture is one of the major seafood supplies worldwide and has caused serious environmental concerns on the coastal zone. Its rapid development has been shown to disrupt the sediment ecosystems and thus influence the benthic bacterial communities. Bacterial diversity and community structure within both adjacent farms and non-cultured zones intertidal sediments along the coasts of Qinhuangdao and Dalian, China, were investigated using full-length 16S rRNA gene-based T-RFLP analyses and clone library construction. Richness and Shannon-Wiener index were significantly increased at sites adjacent the mariculture farm with mean values of 29 and 2.97 from peak profiles of T-RFLP result. Clustering analyses suggested that impacts of mariculture on bacterial diversity of sediment were significantly larger than those resulted from temporal and spatial scales. Upon comparisons of RFLP patterns from 602 clones from libraries of the selected five samples, 137 OTUs were retrieved. Members of gamma- and delta-Proteobacteria, Bacilli, Flavobacteria, and Actinobacteria were recorded in all libraries. In addition, gamma-Proteobacteria were dominant in all samples (21.7 similar to 45.0 %). Redundancy analysis revealed that the distribution of bacterial composition seemed to be determined by the variables of salinity, PO4 (3-)-P, NH4 (+)-N, and Chlorophyll a content. The phyla of gamma-Proteobacteria, Clostridia, Flavobacteria, Bacilli, and Planctomycetes were principal components to contribute to the bacterial differences of clone libraries. Our finding demonstrated that these phyla could display variations of bacterial composition linked to environmental disturbance resulted from mariculture.
学科主题	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Ecology ; Marine & Freshwater Biology ; Microbiology
研究领域[WOS]	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology
关键词[WOS]	AMMONIA-OXIDIZING BACTERIA ; FISH FARM SEDIMENTS ; MICROBIAL COMMUNITY ; ENVIRONMENTAL GRADIENTS ; SPATIAL-DISTRIBUTION ; CHANGJIANG ESTUARY ; SURFACE SEDIMENTS ; CLONE LIBRARIES ; COASTAL WATERS ; SEA
收录类别	SCI
原文出处	10.1007/s00248-013-0272-6
语种	英语
WOS记录号	WOS:000325778500014
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16594]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Tianjin Univ, Sch Environm Sci & Engn, Tianjin 300072, Peoples R China 5.Univ Hawaii Manoa, Dept Microbiol, Honolulu, HI 96822 USA
推荐引用方式 GB/T 7714	Li, Jialin,Li, Fuchao,Yu, Shuxian,et al. Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China[J]. MICROBIAL ECOLOGY,2013,66(4):861-870.
APA	Li, Jialin,Li, Fuchao,Yu, Shuxian,Qin, Song,&Wang, Guangyi.(2013).Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China.MICROBIAL ECOLOGY,66(4),861-870.
MLA	Li, Jialin,et al."Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China".MICROBIAL ECOLOGY 66.4(2013):861-870.