Immunological study of the outer membrane proteins of Vibrio harveyi: Insights that link immunoprotectivity to interference with bacterial infection

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	Immunological study of the outer membrane proteins of Vibrio harveyi: Insights that link immunoprotectivity to interference with bacterial infection
	Yu, Lan-ping 1,2; Hu, Yong-hua 1; Sun, Bo-guang1 ; Sun, Li 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2013-10-01
卷号	35 期号:4 页码:1293-1300
关键词	Vibrio harveyi Outer membrane protein Vaccine Immunoprotectivity Infection
ISSN号	1050-4648
通讯作者	Sun, L
中文摘要	Vibrio harveyi is a bacterial pathogen that affects marine vertebrates and invertebrates. In this study, we identified 13 outer membrane proteins (OMPs) from a pathogenic V. harveyi strain and analyzed their immunological properties. In vivo immunogenicity analysis showed that antibodies specific to recombinant proteins of the 13 OMPs were detected in the antiserum of V. harveyi-infected rat. When used as subunit vaccines to immunize Japanese flounder (Paralichthys olivaceus), all OMPs were able to elicit specific serum antibody production in the vaccinated fish; however, only two OMPs (OMP173 and OMP214) induced high levels (>70%) of relative percent survival. Pre-incubation of V. harveyi with the antisera of protective OMPs significantly impaired bacterial infectivity against peripheral blood leukocytes (PBL), whereas the antisera of non-protective OMPs had no apparent effect on infection. OMP173 antibodies could bind whole V. harveyi cells and exhibit bactericidal effect in a complement-dependent manner. Passive immunization showed that fish received OMP173 antiserum before being infected with V. harveyi exhibited significantly reduced mortality rate and lower bacterial loads in liver, spleen, and kidney. Finally, treatment of FG cells with OMP173 prior to V. harveyi infection protected the cells from bacterial invasion to a significant extent. Take together, these results indicate that two of the examined OMPs induce protective immunity through production of specific antibodies that block bacterial invasion, and that one OMP is likely to be involved in host cell interaction during the infection process. Thus, the immunoprotectivity of the OMPs is probably associated with functional participations of the OMPs in bacterial infection. (C) 2013 Elsevier Ltd. All rights reserved.
英文摘要	Vibrio harveyi is a bacterial pathogen that affects marine vertebrates and invertebrates. In this study, we identified 13 outer membrane proteins (OMPs) from a pathogenic V. harveyi strain and analyzed their immunological properties. In vivo immunogenicity analysis showed that antibodies specific to recombinant proteins of the 13 OMPs were detected in the antiserum of V. harveyi-infected rat. When used as subunit vaccines to immunize Japanese flounder (Paralichthys olivaceus), all OMPs were able to elicit specific serum antibody production in the vaccinated fish; however, only two OMPs (OMP173 and OMP214) induced high levels (>70%) of relative percent survival. Pre-incubation of V. harveyi with the antisera of protective OMPs significantly impaired bacterial infectivity against peripheral blood leukocytes (PBL), whereas the antisera of non-protective OMPs had no apparent effect on infection. OMP173 antibodies could bind whole V. harveyi cells and exhibit bactericidal effect in a complement-dependent manner. Passive immunization showed that fish received OMP173 antiserum before being infected with V. harveyi exhibited significantly reduced mortality rate and lower bacterial loads in liver, spleen, and kidney. Finally, treatment of FG cells with OMP173 prior to V. harveyi infection protected the cells from bacterial invasion to a significant extent. Take together, these results indicate that two of the examined OMPs induce protective immunity through production of specific antibodies that block bacterial invasion, and that one OMP is likely to be involved in host cell interaction during the infection process. Thus, the immunoprotectivity of the OMPs is probably associated with functional participations of the OMPs in bacterial infection. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	FLOUNDER PARALICHTHYS-OLIVACEUS ; SHRIMP LITOPENAEUS-VANNAMEI ; BASS LATES-CALCARIFER ; VACCINE CANDIDATE ; NEISSERIA-MENINGITIDIS ; JAPANESE FLOUNDER ; PENAEUS-MONODON ; PROTECTIVE IMMUNOGEN ; INHIBITORY-ACTIVITY ; SOLEA-SENEGALENSIS
收录类别	SCI
原文出处	10.1016/j.fsi.2013.07.043
语种	英语
WOS记录号	WOS:000324849800027
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16593]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yu, Lan-ping,Hu, Yong-hua,Sun, Bo-guang,et al. Immunological study of the outer membrane proteins of Vibrio harveyi: Insights that link immunoprotectivity to interference with bacterial infection[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(4):1293-1300.
APA	Yu, Lan-ping,Hu, Yong-hua,Sun, Bo-guang,&Sun, Li.(2013).Immunological study of the outer membrane proteins of Vibrio harveyi: Insights that link immunoprotectivity to interference with bacterial infection.FISH & SHELLFISH IMMUNOLOGY,35(4),1293-1300.
MLA	Yu, Lan-ping,et al."Immunological study of the outer membrane proteins of Vibrio harveyi: Insights that link immunoprotectivity to interference with bacterial infection".FISH & SHELLFISH IMMUNOLOGY 35.4(2013):1293-1300.