Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria
	Sun Yumiao 1,2; Li Fuhua 1; Chi Yanhong 1,2; Xiang Jianhai1 ; Xiang, JH
刊名	ACTA OCEANOLOGICA SINICA
	2013-02-01
卷号	32 期号:2 页码:52-58
关键词	WSSV VP28 Exopalamon carincauda live bacterial deliver
ISSN号	0253-505X
通讯作者	Xiang, JH
中文摘要	Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus (WSSV) envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a constitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant protein products outside, DhpVB couldmake VP28 directly interact with the shrimp when injected into shrimp. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI (Resistance index) of 0.67 +/- 0.08, compared with 0.41 +/- 0.09 of bacteria without VP28 (P < 0.05). The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV.
英文摘要	Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus (WSSV) envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a constitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant protein products outside, DhpVB couldmake VP28 directly interact with the shrimp when injected into shrimp. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI (Resistance index) of 0.67 +/- 0.08, compared with 0.41 +/- 0.09 of bacteria without VP28 (P < 0.05). The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV.
学科主题	Oceanography
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Oceanography
研究领域[WOS]	Oceanography
关键词[WOS]	WHITE-SPOT-SYNDROME ; SYNDROME-VIRUS WSSV ; ENVELOPE PROTEIN VP28 ; PENAEUS-MONODON ; PROCAMBARUS-CLARKII ; PROTECTIVE IMMUNITY ; VACCINATION TRIALS ; ORAL VACCINATION ; SUBUNIT VACCINE ; GENOME SEQUENCE
收录类别	SCI
原文出处	10.1007/s13131-013-0261-0
语种	英语
WOS记录号	WOS:000314699900007
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16564]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Xiang, JH
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Sun Yumiao,Li Fuhua,Chi Yanhong,et al. Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria[J]. ACTA OCEANOLOGICA SINICA,2013,32(2):52-58.
APA	Sun Yumiao,Li Fuhua,Chi Yanhong,Xiang Jianhai,&Xiang, JH.(2013).Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria.ACTA OCEANOLOGICA SINICA,32(2),52-58.
MLA	Sun Yumiao,et al."Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria".ACTA OCEANOLOGICA SINICA 32.2(2013):52-58.