Comprehensive Extraction of Agar and R-Phycoerythrin from Gracilaria lemaneiformis (Bangiales, Rhodophyta)

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	Comprehensive Extraction of Agar and R-Phycoerythrin from Gracilaria lemaneiformis (Bangiales, Rhodophyta)
	Niu, Jianfeng 1; Xu, Meiling 3; Wang, Guangce 1,3; Zhang, Kaiyue 4; Peng, Guang 1
刊名	INDIAN JOURNAL OF GEO-MARINE SCIENCES
	2013-02-01
卷号	42 期号:1 页码:21-28
关键词	Gracilaria lemaneiformis R-phycoerythrin expanded bed adsorption ion-exchange chromatography agar
ISSN号	0379-5136
通讯作者	Wang, GC
中文摘要	Water-soluble proteins from Gracilaria lemaneiformis were extracted and further purification for R-phycoerythrin (R-PE) was carried out. Remaining cell mass was used for agar extraction. Approximately 21% of the R-PE recovered from the crude extract and the yield of R-PE with a purification ratio >3.2 were 0,36 mg g(-1) fresh G, lemaneiformis, The yield of agar was 15,2%, with the gel strength of 895 +/- 20 g cm(-2) in a 1% gel at 30 degrees C, which showed no significant difference from those obtained from the directed agar extraction using the dried algae. Research in the experiments also revealed that R-PE could purify from the waste hydration solution of the dried alga, although its recovery and purity found to be lower than when it Was extracted from fresh algae,
英文摘要	Water-soluble proteins from Gracilaria lemaneiformis were extracted and further purification for R-phycoerythrin (R-PE) was carried out. Remaining cell mass was used for agar extraction. Approximately 21% of the R-PE recovered from the crude extract and the yield of R-PE with a purification ratio >3.2 were 0,36 mg g(-1) fresh G, lemaneiformis, The yield of agar was 15,2%, with the gel strength of 895 +/- 20 g cm(-2) in a 1% gel at 30 degrees C, which showed no significant difference from those obtained from the directed agar extraction using the dried algae. Research in the experiments also revealed that R-PE could purify from the waste hydration solution of the dried alga, although its recovery and purity found to be lower than when it Was extracted from fresh algae,
学科主题	Oceanography
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Oceanography
研究领域[WOS]	Oceanography
关键词[WOS]	EXPANDED BED ADSORPTION ; LARGE-SCALE ISOLATION ; GEL-ELECTROPHORESIS ; B-PHYCOERYTHRIN ; RED ALGAE ; PURIFICATION ; PHYCOCYANIN ; RECOVERY ; QUALITY
收录类别	SCI
语种	英语
WOS记录号	WOS:000317453500004
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16550]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Nantong Branch, Nantong 226006, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201303, Peoples R China
推荐引用方式 GB/T 7714	Niu, Jianfeng,Xu, Meiling,Wang, Guangce,et al. Comprehensive Extraction of Agar and R-Phycoerythrin from Gracilaria lemaneiformis (Bangiales, Rhodophyta)[J]. INDIAN JOURNAL OF GEO-MARINE SCIENCES,2013,42(1):21-28.
APA	Niu, Jianfeng,Xu, Meiling,Wang, Guangce,Zhang, Kaiyue,&Peng, Guang.(2013).Comprehensive Extraction of Agar and R-Phycoerythrin from Gracilaria lemaneiformis (Bangiales, Rhodophyta).INDIAN JOURNAL OF GEO-MARINE SCIENCES,42(1),21-28.
MLA	Niu, Jianfeng,et al."Comprehensive Extraction of Agar and R-Phycoerythrin from Gracilaria lemaneiformis (Bangiales, Rhodophyta)".INDIAN JOURNAL OF GEO-MARINE SCIENCES 42.1(2013):21-28.