Characteristics of hydrogen production by an aciduric transposon-mutagenized strain of Pantoea agglomerans BH18

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	Characteristics of hydrogen production by an aciduric transposon-mutagenized strain of Pantoea agglomerans BH18
	Liu, Hongyan 1; Wang, Guangce 1,2
刊名	INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
	2013-10-08
卷号	38 期号:30 页码:13192-13197
关键词	Transposon-mutagenized library Mutant strain Acid tolerance Hydrogen production
ISSN号	0360-3199
通讯作者	Wang, GC
中文摘要	Based on the generation of Pantoea agglomerans BH18 library using Tn7 transposon system, mutant screens were conducted for improvement the ability of hydrogen production of this strain. These mutants were used to test for ability of hydrogen production in the initial pH of 5.0. In contrast to wild type strain BH18, a transposon mutant, named as strain TB108, was screened for high hydrogen-producing capability and acid tolerance in the initial pH of 5.0. The factors required for hydrogen production of the aciduric transposon-mutagenized strain TB108 were determined. The mutant strain TB108 similar as wild type strain BH18 was able to produce hydrogen over a wide range of salt concentration from 0.4% to 6%. Under the marine conditions with the initial pH of 5.0 and glucose concentration of 10 g/L, the total hydrogen production of the mutant TB108 was (1.36 +/- 0.04) mol H-2/mol glucose (mean +/- SE.), increasing by 55% compared with wild type. In addition, the mutant strain TB108 could produce hydrogen using many carbon sources such as fructose, glucose, sucrose, sorbitol and so on. This result demonstrated that the mutant strain with high acid tolerance is beneficial for improvement of hydrogen production. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
英文摘要	Based on the generation of Pantoea agglomerans BH18 library using Tn7 transposon system, mutant screens were conducted for improvement the ability of hydrogen production of this strain. These mutants were used to test for ability of hydrogen production in the initial pH of 5.0. In contrast to wild type strain BH18, a transposon mutant, named as strain TB108, was screened for high hydrogen-producing capability and acid tolerance in the initial pH of 5.0. The factors required for hydrogen production of the aciduric transposon-mutagenized strain TB108 were determined. The mutant strain TB108 similar as wild type strain BH18 was able to produce hydrogen over a wide range of salt concentration from 0.4% to 6%. Under the marine conditions with the initial pH of 5.0 and glucose concentration of 10 g/L, the total hydrogen production of the mutant TB108 was (1.36 +/- 0.04) mol H-2/mol glucose (mean +/- SE.), increasing by 55% compared with wild type. In addition, the mutant strain TB108 could produce hydrogen using many carbon sources such as fructose, glucose, sucrose, sorbitol and so on. This result demonstrated that the mutant strain with high acid tolerance is beneficial for improvement of hydrogen production. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
学科主题	Chemistry ; Electrochemistry ; Energy & Fuels
WOS标题词	Science & Technology ; Physical Sciences ; Technology
类目[WOS]	Chemistry, Physical ; Electrochemistry ; Energy & Fuels
研究领域[WOS]	Chemistry ; Electrochemistry ; Energy & Fuels
关键词[WOS]	BIOHYDROGEN PRODUCTION ; SEWAGE-SLUDGE ; COW DUNG ; IMPROVEMENT ; OVEREXPRESSION ; FERMENTATION ; ISOLATE ; STARCH ; WASTE ; GENE
收录类别	SCI
原文出处	10.1016/j.ijhydene.2013.07.065
语种	英语
WOS记录号	WOS:000326214200008
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16536]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin Key Lab Marine Resources & Chem, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Liu, Hongyan,Wang, Guangce. Characteristics of hydrogen production by an aciduric transposon-mutagenized strain of Pantoea agglomerans BH18[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2013,38(30):13192-13197.
APA	Liu, Hongyan,&Wang, Guangce.(2013).Characteristics of hydrogen production by an aciduric transposon-mutagenized strain of Pantoea agglomerans BH18.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,38(30),13192-13197.
MLA	Liu, Hongyan,et al."Characteristics of hydrogen production by an aciduric transposon-mutagenized strain of Pantoea agglomerans BH18".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 38.30(2013):13192-13197.