Proteomic and metabolomic responses of clam Ruditapes philippinarum to arsenic exposure under different salinities

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Proteomic and metabolomic responses of clam Ruditapes philippinarum to arsenic exposure under different salinities
	Wu, Huifeng1 ; Liu, Xiaoli 1,2; Zhang, Xingyan 1,2; Ji, Chenglong1,2 ; Zhao, Jianmin1 ; Yu, Junbao1
刊名	AQUATIC TOXICOLOGY
	2013-07-15
卷号	136 页码:91-100
关键词	Toxicological effect Salinity Arsenic Ruditapes philippinarum Proteomics Metabolomics
ISSN号	0166-445X
其他题名	Tetra(p-tolyl)borate-Functionalized Solvent Polymeric Membrane A Facile and Sensitive Sensing Platform for Peroxidase and Peroxidase Mimetics.pdf
通讯作者	Wu, HF (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
产权排序	[Wu, Huifeng; Liu, Xiaoli; Zhang, Xingyan; Ji, Chenglong; Zhao, Jianmin; Yu, Junbao] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China; [Liu, Xiaoli; Zhang, Xingyan; Ji, Chenglong] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
中文摘要	Arsenic (As) contamination is a severe problem in the intertidal zones of the Bohai Sea (China) with wide salinity variation. In the present study, we combined proteomics and metabolomics to characterize the differential responses of arsenic in clam Ruditapes philippinarum under different salinities (31.1, 23.3 and 15.6 psu). Both proteomic and metabolomic responses indicated that varying salinities could significantly affect the toxicological responses of clams to As. Metabolic biomarkers revealed that the environmentally relevant arsenic (20 mu g L-1) exposure induced disturbance in energy metabolism and/or osmotic regulation under different salinities, whereas protein biomarkers indicated oxidative stress, cellular injury and apoptosis and disturbance in energy metabolism. In addition, the up-regulated proteins including ATP synthase, succinyl-CoA synthetase and nucleoside diphosphate kinase were validated by related metabolites, succinate and ATP, which confirmed the disturbance in energy metabolism in clam gills at low salinity (15.6 psu). These findings provide important insights into toxicological effects of environmental contaminant at molecular levels using combined proteomics and metabolomics. (C) 2013 Elsevier B.V. All rights reserved.
英文摘要	Arsenic (As) contamination is a severe problem in the intertidal zones of the Bohai Sea (China) with wide salinity variation. In the present study, we combined proteomics and metabolomics to characterize the differential responses of arsenic in clam Ruditapes philippinarum under different salinities (31.1, 23.3 and 15.6 psu). Both proteomic and metabolomic responses indicated that varying salinities could significantly affect the toxicological responses of clams to As. Metabolic biomarkers revealed that the environmentally relevant arsenic (20 mu g L-1) exposure induced disturbance in energy metabolism and/or osmotic regulation under different salinities, whereas protein biomarkers indicated oxidative stress, cellular injury and apoptosis and disturbance in energy metabolism. In addition, the up-regulated proteins including ATP synthase, succinyl-CoA synthetase and nucleoside diphosphate kinase were validated by related metabolites, succinate and ATP, which confirmed the disturbance in energy metabolism in clam gills at low salinity (15.6 psu). These findings provide important insights into toxicological effects of environmental contaminant at molecular levels using combined proteomics and metabolomics. (C) 2013 Elsevier B.V. All rights reserved.
学科主题	Marine & Freshwater Biology ; Toxicology
研究领域[WOS]	Marine & Freshwater Biology ; Toxicology
关键词[WOS]	LASER-DESORPTION/IONIZATION-TIME ; METAL CONTAMINATION ; MYTILUS-EDULIS ; PROTEIN ; NMR ; IDENTIFICATION ; BINDING ; GEL ; BIOACCUMULATION ; SPECTROSCOPY
收录类别	SCI
资助信息	NSFC [41106102, 21237004]
原文出处	http://dx.doi.org/10.1016/j.aquatox.2013.03.020
语种	英语
WOS记录号	WOS:000322057200009
公开日期	2014-07-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6974]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室烟台海岸带研究所_滨海湿地实验室
作者单位	1.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wu, Huifeng,Liu, Xiaoli,Zhang, Xingyan,et al. Proteomic and metabolomic responses of clam Ruditapes philippinarum to arsenic exposure under different salinities[J]. AQUATIC TOXICOLOGY,2013,136:91-100.
APA	Wu, Huifeng,Liu, Xiaoli,Zhang, Xingyan,Ji, Chenglong,Zhao, Jianmin,&Yu, Junbao.(2013).Proteomic and metabolomic responses of clam Ruditapes philippinarum to arsenic exposure under different salinities.AQUATIC TOXICOLOGY,136,91-100.
MLA	Wu, Huifeng,et al."Proteomic and metabolomic responses of clam Ruditapes philippinarum to arsenic exposure under different salinities".AQUATIC TOXICOLOGY 136(2013):91-100.