Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge
	Liu, Xiaoli 1; Zhao, Jianmin2,3 ; Wu, Huifeng2,3 ; Wang, Qing2,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2013-12-01
卷号	35 期号:6 页码:1969-1975
关键词	Metabolomics Ruditapes philippinarum Pedigree Vibrio harveyi Hepatopancreas
ISSN号	1050-4648
通讯作者	Liu, XL (reprint author), Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China. zlylxl2009@gmail.com
产权排序	[Liu, Xiaoli] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; [Zhao, Jianmin; Wu, Huifeng; Wang, Qing] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhao, Jianmin; Wu, Huifeng; Wang, Qing] YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
中文摘要	Manila clam Ruditapes philippinarum is an important marine aquaculture shellfish. This species has several pedigrees including White, Zebra, Liangdao Red and Marine Red distributing in the coastal areas in North China. In this work, we studied the metabolic differences induced by Vibrio harveyi in hepatopancreas from White and Zebra clams using NMR-based metabolomics. Metabolic responses (e.g., amino acids, glucose, glycogen, ATP and succinate) and altered mRNA expression levels of related genes (ATP synthase, heat shock protein 90, defensin and lysozyme) suggested that V harveyi induced clear disruption in energy metabolism and immune stresses in both White and Zebra clam hepatopancreas. However, V. harveyi caused obvious osmotic stress in Zebra clam hepatopancreas, which was not observed in V. harveyi-challenged White clams samples. In addition, V. harveyi challenge induced more severe disruption in energy metabolism and immune stress in White clams than in Zebra clams. Overall, our results indicated that the biological differences between different pedigrees of R. philippinarum should be considered in immunity studies. (C) 2013 Elsevier Ltd. All rights reserved.
英文摘要	Manila clam Ruditapes philippinarum is an important marine aquaculture shellfish. This species has several pedigrees including White, Zebra, Liangdao Red and Marine Red distributing in the coastal areas in North China. In this work, we studied the metabolic differences induced by Vibrio harveyi in hepatopancreas from White and Zebra clams using NMR-based metabolomics. Metabolic responses (e.g., amino acids, glucose, glycogen, ATP and succinate) and altered mRNA expression levels of related genes (ATP synthase, heat shock protein 90, defensin and lysozyme) suggested that V harveyi induced clear disruption in energy metabolism and immune stresses in both White and Zebra clam hepatopancreas. However, V. harveyi caused obvious osmotic stress in Zebra clam hepatopancreas, which was not observed in V. harveyi-challenged White clams samples. In addition, V. harveyi challenge induced more severe disruption in energy metabolism and immune stress in White clams than in Zebra clams. Overall, our results indicated that the biological differences between different pedigrees of R. philippinarum should be considered in immunity studies. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	NMR METABOLOMICS ; INVERTEBRATES ; VERTEBRATES ; CHAIN
收录类别	SCI
原文出处	http://dx.doi.org/10.1016/j.fsi.2013.09.037
语种	英语
WOS记录号	WOS:000330158800032
公开日期	2014-07-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6928]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 3.YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xiaoli,Zhao, Jianmin,Wu, Huifeng,et al. Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(6):1969-1975.
APA	Liu, Xiaoli,Zhao, Jianmin,Wu, Huifeng,&Wang, Qing.(2013).Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge.FISH & SHELLFISH IMMUNOLOGY,35(6),1969-1975.
MLA	Liu, Xiaoli,et al."Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge".FISH & SHELLFISH IMMUNOLOGY 35.6(2013):1969-1975.