How well parameterization will improve gross primary production (GPP) estimation using the MODerate-resolution Imaging Spectroradiometer (MODIS) algorithm has been rarely investigated. We adjusted the parameters in the algorithm for 21 selected eddy-covariance flux towers which represented nine typical plant functional types (PFTs). We then compared these estimates of the MOD17A2 product, by the MODIS algorithm with default parameters in the Biome Property Look-Up Table, and by a two-leaf Farquhar model. The results indicate that optimizing the maximum light use efficiency ((max)) in the algorithm would improve GPP estimation, especially for deciduous vegetation, though it could not compensate the underestimation during summer caused by the one-leaf upscaling strategy. Adding the soil water factor to the algorithm would not significantly affect performance, but it could make the adjusted (max) more robust for sites with the same PFT and among different PFTs. Even with adjusted parameters, both one-leaf and two-leaf models would not capture seasonally photosynthetic dynamics, thereby we suggest that further improvement in GPP estimaiton is required by taking into consideration seasonal variations of the key parameters and variables.