Effects of tris(1,3-dichloro-2-propyl) phosphate and triphenyl phosphate on receptor-associated mRNA expression in zebrafish embryos/larvae

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Effects of tris(1,3-dichloro-2-propyl) phosphate and triphenyl phosphate on receptor-associated mRNA expression in zebrafish embryos/larvae
	Liu, Chunsheng ; Wang, Qiangwei ; Liang, Kang ; Liu, Jingfu ; Zhou, Bingsheng ; Zhang, Xiaowei ; Liu, Hongling ; Giesy, John P. ; Yu, Hongxia
刊名	AQUATIC TOXICOLOGY
	2013
卷号	128 期号:1 页码:147-157
通讯作者	Liu, CS
中文摘要	Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) and triphenyl phosphate (TPP) are frequently detected in biota, including fish. However, knowledge of the toxicological and molecular effects of these currently used flame retardants is limited. In the present study, an in vivo screening approach was developed to evaluate effects of TDCPP and TPP on developmental endpoints and receptor-associated expression of mRNA in zebrafish embryos/larvae. Exposure to TDCPP or TPP resulted in significantly smaller rates of hatching and survival, in dose- and time-dependent manners. The median lethal concentration (LC50) was 7.0 mg/L for TDCPP and 29.6 mg/L for TPP at 120 hour post-fertilization (hpf). Real-time PCR revealed alterations in expression of mRNAs involved in aryl hydrocarbon receptors (AhRs)-, peroxisome proliferator-activated receptor alpha (PPAR alpha)-, estrogenic receptors (ERs)-, thyroid hormone receptor alpha (TR alpha)-, glucocorticoid receptor (GR)-, and mineralocorticoid receptor (MR)-centered gene networks. Exposure to positive control chemicals significantly altered abundances of mRNA in corresponding receptor-centered gene networks, a result that suggests that it is feasible to use zebrafish embryos/larvae to evaluate effects of chemicals on mRNA expression in these gene networks. Exposure to TDCPP altered transcriptional profiles in all six receptor-centered gene networks, thus exerting multiple toxic effects. TPP was easily metabolized and its potency to change expression of mRNA involved in receptor-centered gene networks was weaker than that of TDCPP. The PPAR alpha- and TR alpha-centered gene networks might be the primary pathways affected by TPP. Taken together, these results demonstrated that TDCPP and TPP could alter mRNA expression of genes involved in the six receptor-centered gene networks in zebrafish embryos/larvae, and TDCPP seemed to have higher potency in changing the mRNA expression of these genes. (c) 2012 Elsevier B.V. All rights reserved.
收录类别	SCI
WOS记录号	WOS:000316531900015
公开日期	2014-06-06
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/7520]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Liu, Chunsheng,Wang, Qiangwei,Liang, Kang,et al. Effects of tris(1,3-dichloro-2-propyl) phosphate and triphenyl phosphate on receptor-associated mRNA expression in zebrafish embryos/larvae[J]. AQUATIC TOXICOLOGY,2013,128(1):147-157.
APA	Liu, Chunsheng.,Wang, Qiangwei.,Liang, Kang.,Liu, Jingfu.,Zhou, Bingsheng.,...&Yu, Hongxia.(2013).Effects of tris(1,3-dichloro-2-propyl) phosphate and triphenyl phosphate on receptor-associated mRNA expression in zebrafish embryos/larvae.AQUATIC TOXICOLOGY,128(1),147-157.
MLA	Liu, Chunsheng,et al."Effects of tris(1,3-dichloro-2-propyl) phosphate and triphenyl phosphate on receptor-associated mRNA expression in zebrafish embryos/larvae".AQUATIC TOXICOLOGY 128.1(2013):147-157.