Assessing genetic structure, diversity of bacterial aerosol from aeration system in an oxidation ditch wastewater treatment plant by culture methods and bio-molecular tools

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	Assessing genetic structure, diversity of bacterial aerosol from aeration system in an oxidation ditch wastewater treatment plant by culture methods and bio-molecular tools
	Li, Lin ; Han, Yunping ; Liu, Junxin
刊名	ENVIRONMENTAL MONITORING AND ASSESSMENT
	2013
卷号	185 期号:1 页码:603-613
通讯作者	Liu, JX
中文摘要	Airborne bacteria emissions from oxidation ditch with rotating aeration brushes were investigated in a municipal wastewater treatment plant in Beijing, China. Microbial samples were collected at different distances from the rotating brushes, different heights above the water surface, and different operation state over a 3-month period (April, May, and June) in order to estimate the seasonal variation and site-related distribution characteristics of the microorganisms present. The concentration of bacterial aerosol was analyzed by culture methods, while their dominant species, genetic structure and diversity were assayed using bio-molecular tools. Results showed that total microbial concentrations were highest in June and lowest in April. The mechanical rotation caused remarkable variation in concentration and diversity of culturable airborne bacteria before and after the rotating brushes. The highest concentration was observed near the rotating brushes (931 +/- 129-3,952 +/- 730 CFU/m(3)), with concentration decreasing as distance and height increased. Bacterial community polymerase chain reaction and denaturing gradient gel electrophoresis indicated that diversity decreased gradually with increasing height above the water surface but remained relatively constant at the same height. All dominant bacteria identified by DNA sequence analysis belonged to Firmicutes. Pathogenic species such as Moraxella nonliquefaciens and Flavobacterium odoratum were isolated from the bioaerosols. Due to the serious health risks involved, exposure of sewage workers to airborne microorganisms caused by brush aerators should be monitored and controlled.
收录类别	SCI
WOS记录号	WOS:000312835300050
公开日期	2014-06-06
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/7356]
专题	生态环境研究中心_水污染控制实验室
推荐引用方式 GB/T 7714	Li, Lin,Han, Yunping,Liu, Junxin. Assessing genetic structure, diversity of bacterial aerosol from aeration system in an oxidation ditch wastewater treatment plant by culture methods and bio-molecular tools[J]. ENVIRONMENTAL MONITORING AND ASSESSMENT,2013,185(1):603-613.
APA	Li, Lin,Han, Yunping,&Liu, Junxin.(2013).Assessing genetic structure, diversity of bacterial aerosol from aeration system in an oxidation ditch wastewater treatment plant by culture methods and bio-molecular tools.ENVIRONMENTAL MONITORING AND ASSESSMENT,185(1),603-613.
MLA	Li, Lin,et al."Assessing genetic structure, diversity of bacterial aerosol from aeration system in an oxidation ditch wastewater treatment plant by culture methods and bio-molecular tools".ENVIRONMENTAL MONITORING AND ASSESSMENT 185.1(2013):603-613.