Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata
	Cheng, Bao-Hui 1,2; Chan, Judy Yuet-Wa 1,2; Chan, Ben Chung-Lap 1,2; Lin, Huang-Quan 1,2; Han, Xiao-Qiang 1,2; Zhou, Xuelin 1,2; Wan, David Chi-Cheong 3; Wang, Yi-Fen4 ; Leung, Ping-Chung 1,2; Fung, Kwok-Pui 1,2,3
刊名	CARBOHYDRATE POLYMERS
	2014-03-15
卷号	103 页码:244-249
关键词	Houttuynia cordata Pectic polysaccharide HCP-2 Structural characterization Immunomodulation
ISSN号	0144-8617
通讯作者	Bik-San Lau, C (reprint author), Chinese Univ Hong Kong, Inst Chinese Med, E305,Sci Ctr East Block, Shatin, Hong Kong, Peoples R China. ; claralau@cuhk.edu.hk
英文摘要	Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000 Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-400 HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked alpha-D-galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), macrophage inhibitory protein-1 alpha (MIP-1 alpha), macrophage inhibitory protein-1 beta (MIP-1 beta), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
类目[WOS]	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
研究领域[WOS]	Chemistry ; Polymer Science
关键词[WOS]	PECTIC POLYSACCHARIDES ; ACIDIC POLYSACCHARIDE ; LIPOPOLYSACCHARIDE ; HOMOGALACTURONAN ; ACTIVATION ; EXTRACT ; CELLS ; MICE
收录类别	SCI
语种	英语
WOS记录号	WOS:000332812600033
公开日期	2014-05-16
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/18049]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Univ Hong Kong, Inst Chinese Med, Shatin, Hong Kong, Peoples R China 2.Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources West Ch, Shatin, Hong Kong, Peoples R China 3.Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Shatin, Hong Kong, Peoples R China 4.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming 650201, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Bao-Hui,Chan, Judy Yuet-Wa,Chan, Ben Chung-Lap,et al. Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata[J]. CARBOHYDRATE POLYMERS,2014,103:244-249.
APA	Cheng, Bao-Hui.,Chan, Judy Yuet-Wa.,Chan, Ben Chung-Lap.,Lin, Huang-Quan.,Han, Xiao-Qiang.,...&Bik-San Lau, Clara.(2014).Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata.CARBOHYDRATE POLYMERS,103,244-249.
MLA	Cheng, Bao-Hui,et al."Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata".CARBOHYDRATE POLYMERS 103(2014):244-249.