Isolation and Characterization of cDNAs Encoding Leucoanthocyanidin Reductase and Anthocyanidin Reductase from Populus trichocarpa

	Isolation and Characterization of cDNAs Encoding Leucoanthocyanidin Reductase and Anthocyanidin Reductase from Populus trichocarpa
	Wang, Lijun 1,2; Jiang, Yuanzhong 1; Yuan, Li 1; Lu, Wanxiang 2,3; Yang, Li 1; Karim, Abdul 1; Luo, Keming 1,2,3
刊名	plos one
	2013-05-31
卷号	8 期号:5
ISSN号	1932-6203
通讯作者	luo, km (reprint author), southwest univ, sch life sci, inst resources bot, minist educ,key lab ecoenvironm three gorges rese, chongqing, peoples r china.
中文摘要	proanthocyanidins (pas) contribute to poplar defense mechanisms against biotic and abiotic stresses. transcripts of pa biosynthetic genes accumulated rapidly in response to infection by the fungus marssonina brunnea f.sp. multigermtubi, treatments of salicylic acid (sa) and wounding, resulting in pa accumulation in poplar leaves. anthocyanidin reductase (anr) and leucoanthocyanidin reductase (lar) are two key enzymes of the pa biosynthesis that produce the main subunits: (+)-catechin and (-)-epicatechin required for formation of pa polymers. in populus, anr and lar are encoded by at least two and three highly related genes, respectively. in this study, we isolated and functionally characterized genes ptranr1 and ptrlar1 from p. trichocarpa. phylogenetic analysis shows that populus anr1 and lar1 occurr in two distinct phylogenetic lineages, but both genes have little difference in their tissue distribution, preferentially expressed in roots. overexpression of ptranr1 in poplar resulted in a significant increase in pa levels but no impact on catechin levels. antisense down-regulation of ptranr1 showed reduced pa accumulation in transgenic lines, but increased levels of anthocyanin content. ectopic expression of ptrlar1 in poplar positively regulated the biosynthesis of pas, whereas the accumulation of anthocyanin and flavonol was significantly reduced (p<0.05) in all transgenic plants compared to the control plants. these results suggest that both ptranr1 and ptrlar1 contribute to pa biosynthesis in populus.
英文摘要	proanthocyanidins (pas) contribute to poplar defense mechanisms against biotic and abiotic stresses. transcripts of pa biosynthetic genes accumulated rapidly in response to infection by the fungus marssonina brunnea f.sp. multigermtubi, treatments of salicylic acid (sa) and wounding, resulting in pa accumulation in poplar leaves. anthocyanidin reductase (anr) and leucoanthocyanidin reductase (lar) are two key enzymes of the pa biosynthesis that produce the main subunits: (+)-catechin and (-)-epicatechin required for formation of pa polymers. in populus, anr and lar are encoded by at least two and three highly related genes, respectively. in this study, we isolated and functionally characterized genes ptranr1 and ptrlar1 from p. trichocarpa. phylogenetic analysis shows that populus anr1 and lar1 occurr in two distinct phylogenetic lineages, but both genes have little difference in their tissue distribution, preferentially expressed in roots. overexpression of ptranr1 in poplar resulted in a significant increase in pa levels but no impact on catechin levels. antisense down-regulation of ptranr1 showed reduced pa accumulation in transgenic lines, but increased levels of anthocyanin content. ectopic expression of ptrlar1 in poplar positively regulated the biosynthesis of pas, whereas the accumulation of anthocyanin and flavonol was significantly reduced (p<0.05) in all transgenic plants compared to the control plants. these results suggest that both ptranr1 and ptrlar1 contribute to pa biosynthesis in populus.
WOS标题词	science & technology
类目[WOS]	multidisciplinary sciences
研究领域[WOS]	science & technology - other topics
关键词[WOS]	proanthocyanidin synthesis ; flavonoid biosynthesis ; transcription factor ; lotus-corniculatus ; molecular-cloning ; condensed tannins ; expression ; gene ; biochemistry ; pathways
收录类别	SCI
语种	英语
WOS记录号	WOS:000319799900075
公开日期	2014-05-09
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/3939]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Southwest Univ, Sch Life Sci, Inst Resources Bot, Minist Educ,Key Lab Ecoenvironm Three Gorges Rese, Chongqing, Peoples R China 2.Southwest Univ, Coll Hort & Landscape Architecture, Chongqing, Peoples R China 3.Chinese Acad Sci, NorthWest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China
推荐引用方式 GB/T 7714	Wang, Lijun,Jiang, Yuanzhong,Yuan, Li,et al. Isolation and Characterization of cDNAs Encoding Leucoanthocyanidin Reductase and Anthocyanidin Reductase from Populus trichocarpa[J]. plos one,2013,8(5).
APA	Wang, Lijun.,Jiang, Yuanzhong.,Yuan, Li.,Lu, Wanxiang.,Yang, Li.,...&Luo, Keming.(2013).Isolation and Characterization of cDNAs Encoding Leucoanthocyanidin Reductase and Anthocyanidin Reductase from Populus trichocarpa.plos one,8(5).
MLA	Wang, Lijun,et al."Isolation and Characterization of cDNAs Encoding Leucoanthocyanidin Reductase and Anthocyanidin Reductase from Populus trichocarpa".plos one 8.5(2013).