Nanoscale imaging and mechanical analysis of Fc receptor-mediated macrophage phagocytosis against cancer cells

CORC > 沈阳自动化研究所 > 中国科学院沈阳自动化研究所 > 机器人学研究室

	Nanoscale imaging and mechanical analysis of Fc receptor-mediated macrophage phagocytosis against cancer cells
	Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Xiao XB(肖秀斌); Zhang WJ(张伟京)
刊名	Langmuir
	2014
卷号	30 期号:6 页码:1609-1621
关键词	Antigen-antibody reactions Atomic force microscopy Biomechanics Fluorescence Fluorescence microscopy Mechanical properties Nanotechnology Optical data storage Optical microscopy
ISSN号	0743-7463
通讯作者	刘连庆 ; 席宁
产权排序	1
中文摘要	Fc receptor-mediated macrophage phagocytosis against cancer cells is an important mechanism in the immune therapy of cancers. Traditional research about macrophage phagocytosis was based on optical microscopy, which cannot reveal detailed information because of the 200-nm-resolution limit. Quantitatively investigating the macrophage phagocytosis at micro- and nanoscale levels is still scarce. The advent of atomic force microscopy (AFM) offers an excellent analytical instrument for quantitatively investigating the biological processes at single-cell and single-molecule levels under native conditions. In this work, we combined AFM and fluorescence microscopy to visualize and quantify the detailed changes in cell morphology and mechanical properties during the process of Fc receptor-mediated macrophage phagocytosis against cancer cells. Lymphoma cells were discernible by fluorescence staining. Then, the dynamic process of phagocytosis was observed by time-lapse optical microscopy. Next, AFM was applied to investigate the detailed cellular behaviors during macrophage phagocytosis under the guidance of fluorescence recognition. AFM imaging revealed the distinct features in cellular ultramicrostructures for the different steps of macrophage phagocytosis. AFM cell mechanical property measurements indicated that the binding of cancer cells to macrophages could make macrophages become stiffer. The experimental results provide novel insights in understanding the Fc-receptor-mediated macrophage phagocytosis.
WOS标题词	Science & Technology ; Physical Sciences ; Technology
类目[WOS]	Chemistry, Multidisciplinary ; Chemistry, Physical ; Materials Science, Multidisciplinary
研究领域[WOS]	Chemistry ; Materials Science
关键词[WOS]	ATOMIC-FORCE MICROSCOPY ; ANTIBODY-BASED IMMUNOTHERAPY ; MONOCLONAL-ANTIBODIES ; LIVING CELLS ; MOTILE CELLS ; AFM ; CYTOSKELETON ; INFECTION ; TARGET ; HETEROGENEITY
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000331774400017
内容类型	期刊论文
源URL	[http://ir.sia.cn/handle/173321/14728]
专题	沈阳自动化研究所_机器人学研究室
推荐引用方式 GB/T 7714	Li M,Liu LQ,Xi N,et al. Nanoscale imaging and mechanical analysis of Fc receptor-mediated macrophage phagocytosis against cancer cells[J]. Langmuir,2014,30(6):1609-1621.
APA	Li M,Liu LQ,Xi N,Wang YC,Xiao XB,&Zhang WJ.(2014).Nanoscale imaging and mechanical analysis of Fc receptor-mediated macrophage phagocytosis against cancer cells.Langmuir,30(6),1609-1621.
MLA	Li M,et al."Nanoscale imaging and mechanical analysis of Fc receptor-mediated macrophage phagocytosis against cancer cells".Langmuir 30.6(2014):1609-1621.