Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR.

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 植物代谢工程团队

	Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR.
	Chi, X. ; Hu, R. ; Yang, Q. ; Zhang, X. ; Pan, L. ; Chen, N. ; . . . Yu, S.
刊名	Mol Genet Genomics
	2012
卷号	287 期号:2 页码:167-176
关键词	Peanut qRT-PCR Reference Genes Validation
中文摘要	Abstract Quantitative real-time reverse transcription PCR (qRT-PCR), a sensitive technique for quantifying gene expression, depends on the stability of the reference gene(s) used for data normalization. Only a few studies on the reference genes have been done with peanut to date. In the present study, 14 potential reference genes in peanut were evaluated for their expression stability using the geNorm and NormFinder statistical algorithms. Expression stability was assessed by qRT-PCR across 32 biological samples, including various tissue types, seed developmental stages, salt and cold treatments. The results showed that the best-ranked references genes diVered across the samples. UKN1, UKN2, TUA5 and ACT11 were the most stable across all the tested samples. A combination of ACT11, TUA5, UKN2, PEPKR1 and TIP41 would be appropriate as a reference panel for normalizing gene expression data across the various tissues tested, whereas the combination of TUA5 and UKN1 was the most suitable for seed developmental stages. TUA5 and EF1b exhibited the most stable expression under cold treatment. For salt-treated leaves, TUA5 and UKN2 were the most stably expressed and HDC and UKN1 for salt-treated roots. The relative gene expression level of peanut Cys2/His2-type zinc Wnger protein gene AhZFP1 was analyzed in order to validate the reference genes selected for this study. These results provide guidelines for the selection of reference genes under diVerent experimental conditions and also a foundation for more accurate and widespread use of qRT-PCR in peanut gene analysis.
学科主题	能源植物资源
收录类别	SCI
语种	英语
公开日期	2014-03-27
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1740]
专题	青岛生物能源与过程研究所_植物代谢工程团队
推荐引用方式 GB/T 7714	Chi, X.,Hu, R.,Yang, Q.,et al. Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR.[J]. Mol Genet Genomics,2012,287(2):167-176.
APA	Chi, X..,Hu, R..,Yang, Q..,Zhang, X..,Pan, L..,...&. . . Yu, S..(2012).Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR..Mol Genet Genomics,287(2),167-176.
MLA	Chi, X.,et al."Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR.".Mol Genet Genomics 287.2(2012):167-176.