Biosynthetic pathway for poly(3-Hydroxypropionate) in recombinant Escherichia coli

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Biosynthetic pathway for poly(3-Hydroxypropionate) in recombinant Escherichia coli
	Wang, Qi 1,2; Liu, Changshui 1,2; Xian, Mo1 ; Zhang, Yongguang 1; Zhao, Guang 1
刊名	JOURNAL OF MICROBIOLOGY
	2012-08-01
卷号	50 期号:4 页码:693-697
关键词	poly(3-hydroxypropionate) malonyl-CoA reductase propionyl-coenzyme A synthetase polyhydroxyalkanoate synthase recombinant E. coli
英文摘要	Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, we engineered a P3HP biosynthetic pathway in recombinant Escherichia coli. The genes for malonyl-CoA reductase (mcr, from Chloroflexus aurantiacus), propionyl-CoA synthetase (prpE, from E. coli), and polyhydroxyalkanoate synthase (phaC1, from Ralstonia eutropha) were cloned and expressed in E. coli. The E. coli genes accABCD encoding acetyl-CoA carboxylase were used to channel the carbon into the P3HP pathway. Using glucose as a sole carbon source, the cell yield and P3HP content were 1.32 g/L and 0.98% (wt/wt [cell dry weight]), respectively. Although the yield is relatively low, our study shows the feasibility of engineering a P3HP biosynthetic pathway using a structurally unrelated carbon source in bacteria.
学科主题	生物基化学品
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	PSEUDOMONAS-OLEOVORANS ; RALSTONIA-EUTROPHA ; POLYESTERS ; COENZYME ; POLYHYDROXYALKANOATES ; 3-HYDROXYPROPIONATE ; ACCUMULATION ; SYNTHETASE ; PUTIDA ; ACID
收录类别	SCI
语种	英语
WOS记录号	WOS:000307996700022
公开日期	2014-03-27
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1749]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qi,Liu, Changshui,Xian, Mo,et al. Biosynthetic pathway for poly(3-Hydroxypropionate) in recombinant Escherichia coli[J]. JOURNAL OF MICROBIOLOGY,2012,50(4):693-697.
APA	Wang, Qi,Liu, Changshui,Xian, Mo,Zhang, Yongguang,&Zhao, Guang.(2012).Biosynthetic pathway for poly(3-Hydroxypropionate) in recombinant Escherichia coli.JOURNAL OF MICROBIOLOGY,50(4),693-697.
MLA	Wang, Qi,et al."Biosynthetic pathway for poly(3-Hydroxypropionate) in recombinant Escherichia coli".JOURNAL OF MICROBIOLOGY 50.4(2012):693-697.