Boosting the free fatty acid synthesis of Escherichia coli by expression of a cytosolic Acinetobacter baylyi thioesterase

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Boosting the free fatty acid synthesis of Escherichia coli by expression of a cytosolic Acinetobacter baylyi thioesterase
	Zheng, Yanning 1,2; Li, Lingling 1,3; Liu, Qiang 1,3; Qin, Wen 3; Yang, Jianming 1,2; Cao, Yujin 1; Jiang, Xinglin 1,2; Zhao, Guang 1; Xian, Mo1
刊名	BIOTECHNOLOGY FOR BIOFUELS
	2012-10-11
卷号	5 期号:1 页码:76
关键词	Thioesterase Acinetobacter baylyi Escherichia coli Free fatty acid Substrate specificity Active-site residues
中文摘要	Abstract Background With the increasing stress from oil price and environmental pollution, aroused attention has been paid to the microbial production of chemicals from renewable sources. The C12/14 and C16/18 alcohols are important feedstocks for the production of surfactants and detergents,which are widely used in the most respected consumer detergents, cleaning products and personal care products worldwide. Though bioproduction of fatty alcohols has been carried out in engineered E. coli, several key problems have not been solved in earlier studies, such as the quite low production of C16/18 alcohol, the lack of optimization of the fatty alcohol biosynthesis pathway, and the uncharacterized performance of the engineered strains in scaled-up system. Results We improved the fatty alcohol production by systematically optimizing the fatty alcohol biosynthesis pathway, mainly targeting three key steps from fatty acyl-acyl carrier proteins (ACPs) to fatty alcohols, which are sequentially catalyzed by thioesterase, acyl-coenzyme A (CoA) synthase and fatty acyl-CoA reductase. By coexpression of thioesterase gene BTE, acyl-CoA synthase gene fadD and fatty acyl-CoA reductase gene acr1, 210.1 mg/L C12/14 alcohol was obtained. A further optimization of expression level of BTE, fadD and acr1 increased the C12/14 alcohol production to 449.2 mg/L, accounting for 75.0% of the total fatty alcohol production (598.6 mg/L). In addition, by coexpression of thioesterase gene‘tesA, acyl-CoA synthase gene fadD and fatty acyl-CoA reductase gene FAR, 101.5 mg/L C16/18 alcohol was obtained, with C16/18 alcohol accounting for 89.2% of the total fatty alcohol production. Conclusions To our knowledge, this is the first report on selective production of C12/14 and C16/18 alcohols by microbial fermentation. This work achieved high-specificity production of both C12/14 and C16/18 alcohols. The encouraging 598.6 mg/L of fatty alcohols represents the highest titer reported so far. In addition, the 101.5 mg/L 89.2% C16/18 alcohol suggests an important breakthrough in C16/18 alcohol production. A more detailed optimization of the expression level of fatty alcohol biosynthesis pathway may contribute to a further improvement of fatty alcohol production.
英文摘要	Background: Thioesterases remove the fatty acyl moiety from the fatty acyl-acyl carrier proteins (ACPs), releasing them as free fatty acids (FFAs), which can be further used to produce a variety of fatty acid-based biofuels, such as biodiesel, fatty alcohols and alkanes. Thioesterases play a key role in the regulation of the fatty acid synthesis in Escherichia coli. Therefore, exploring more promising thioesterases will contribute to the development of industrial microbial lipids production.
学科主题	生物基化学品
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]	Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	CARRIER PROTEIN THIOESTERASE ; SITE-DIRECTED MUTAGENESIS ; ACYL-ACP THIOESTERASES ; SIGNAL PEPTIDES ; BIOSYNTHESIS ; OVERPRODUCTION ; IDENTIFICATION ; CYANOBACTERIA ; SPECIFICITY ; PREDICTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000312708400001
公开日期	2014-03-27
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1747]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Sichuan Agr Univ, Coll Food Sci, Yaan 625014, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Yanning,Li, Lingling,Liu, Qiang,et al. Boosting the free fatty acid synthesis of Escherichia coli by expression of a cytosolic Acinetobacter baylyi thioesterase[J]. BIOTECHNOLOGY FOR BIOFUELS,2012,5(1):76.
APA	Zheng, Yanning.,Li, Lingling.,Liu, Qiang.,Qin, Wen.,Yang, Jianming.,...&Xian, Mo.(2012).Boosting the free fatty acid synthesis of Escherichia coli by expression of a cytosolic Acinetobacter baylyi thioesterase.BIOTECHNOLOGY FOR BIOFUELS,5(1),76.
MLA	Zheng, Yanning,et al."Boosting the free fatty acid synthesis of Escherichia coli by expression of a cytosolic Acinetobacter baylyi thioesterase".BIOTECHNOLOGY FOR BIOFUELS 5.1(2012):76.