A novel colorimetric biosensor for monitoring and detecting acute toxicity in water
Zhai JF ; Yong DM ; Li J ; Dong SJ
刊名analyst
2013
卷号138期号:2页码:702-707
关键词ACTIVATED-SLUDGE PRUSSIAN BLUE NEUTRAL RED ESCHERICHIA-COLI FERRIC PHOSPHATE CELL VIABILITY WASTE-WATER ASSAY IMMOBILIZATION NANOPARTICLES
ISSN号0003-2654
通讯作者dong sj
中文摘要this work presents a new colorimetric microorganism biosensor for monitoring and detecting acute toxicity in water, where prussian blue (pb) is used as the colorimetric indicator and e. coli as the model bacterial. in this biosensor, the electron mediator, ferricyanide, accepts electrons from e. coli during respiration to produce ferrocyanide, which subsequently reacts with ferric ions to yield pb, a famous material with a blue color. since toxicants can inhibit the respiratory activity of e. coli and then reduce the ferrocyanide and consequent pb production, toxicity can be easily detected by measuring the decrease in the production of pb induced by toxicants. three important toxicants, 3,5-dichlorophenol (dcp), as3+, cr6+ are tested and the detection limits are 3.2, 25, and 3.2 ppm, respectively. moreover, we could identify the yellow green to dark green color change by naked eye even at concentrations as low as 12.5 ppm for both dcp and cr6+. subsequently, the acute toxicities of groundwater and south lake water are successfully determined by this sensor. this biosensor is rapid, sensitive and cost-effective, and can thus be regarded as a promising biosensor for giving an early warning of acute water toxicity.
收录类别SCI收录期刊论文
语种英语
WOS记录号WOS:000312074300042
公开日期2014-04-18
内容类型期刊论文
源URL[http://ir.ciac.jl.cn/handle/322003/50443]  
专题长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
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GB/T 7714
Zhai JF,Yong DM,Li J,et al. A novel colorimetric biosensor for monitoring and detecting acute toxicity in water[J]. analyst,2013,138(2):702-707.
APA Zhai JF,Yong DM,Li J,&Dong SJ.(2013).A novel colorimetric biosensor for monitoring and detecting acute toxicity in water.analyst,138(2),702-707.
MLA Zhai JF,et al."A novel colorimetric biosensor for monitoring and detecting acute toxicity in water".analyst 138.2(2013):702-707.
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