Application of ionic liquid as additive in determination of three beta-agonists by capillary electrophoresis with amperometric detection

	Application of ionic liquid as additive in determination of three beta-agonists by capillary electrophoresis with amperometric detection
	Li LB ; Du HW ; Yu H ; Xu L ; You TY
刊名	electrophoresis
	2013
卷号	34 期号:2 页码:277-283
关键词	CHEMILUMINESCENCE DETECTION ELECTROCHEMICAL DETECTION ZONE-ELECTROPHORESIS MASS-SPECTROMETRY ADRENERGIC AGONISTS HUMAN URINE ILLEGAL USE CLENBUTEROL SEPARATION SALBUTAMOL
ISSN号	0173-0835
通讯作者	you ty
中文摘要	ce coupled with amperometric detection method was developed using ionic liquid 1-ethyl-3-methylimidazolium tetrafluoroborate (emimbf4) as additive for the simultaneous detection of clenbuterol (clb), terbutaline (ter), and ractopamine (rac) in feed. the effects of detection potential, concentration of emimbf4, ph, and concentration of the running buffer, separation voltage as well as injection time on the separation and detection of these three beta-agonists were investigated in detail. under the optimum conditions: the detection potential at 1.05 v, 50 mmol/l tris-hac at ph 8.0 with 0.6% (v/v) emimbf4, electrokinetic injection 6 s at 16 kv and separation voltage at 16 kv, a baseline separation for these three analytes could be achieved within 11 min. introduction of emimbf4 into the running buffer resulted in significant improvement in separation selectivity and enhancement in peak currents for those beta-agonists, especially for ter and rac, which could not be separated in the running buffer without additive. the method exhibited wide linear range with lod (s/n = 3) of 2, 1, and 2 nmol/l for clb, ter, and rac, respectively. the precision was determined in both intraday (n = 5) and interday (n = 3) assays, and the rsds for both migration time and peak current were less than 6%. the proposed method was also applied to analyze beta-agonists in feed sample.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000313690100009
公开日期	2014-04-18
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/50395]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Li LB,Du HW,Yu H,et al. Application of ionic liquid as additive in determination of three beta-agonists by capillary electrophoresis with amperometric detection[J]. electrophoresis,2013,34(2):277-283.
APA	Li LB,Du HW,Yu H,Xu L,&You TY.(2013).Application of ionic liquid as additive in determination of three beta-agonists by capillary electrophoresis with amperometric detection.electrophoresis,34(2),277-283.
MLA	Li LB,et al."Application of ionic liquid as additive in determination of three beta-agonists by capillary electrophoresis with amperometric detection".electrophoresis 34.2(2013):277-283.