A novel dark-field microscopy technique coupled with capillary electrophoresis for visual analysis of single nanoparticles

	A novel dark-field microscopy technique coupled with capillary electrophoresis for visual analysis of single nanoparticles
	Li LB ; Yu H ; Liu D ; You TY
刊名	analyst
	2013
卷号	138 期号:13 页码:3705-3710
关键词	INDUCED FLUORESCENCE DETECTION GOLD NANOPARTICLES SILVER NANOPARTICLES ZONE-ELECTROPHORESIS METAL NANOPARTICLES SEPARATION PLASMON PARTICLES SIZE CELLS
ISSN号	0003-2654
通讯作者	you ty
中文摘要	a system for interfacing dark field microscopy (dfm) with capillary electrophoresis (ce) was demonstrated and applied to investigate the movement of single gold nanoparticles (aunps) visually and in real time for the first time. when using similar to 60 nm cetyl trimethyl ammonium bromide (ctab) and sodium dodecyl sulfate (sds) capped aunps as target analytes, preliminary data indicated that differently charged aunps had different movement velocities (v). from the result of the effect of applied voltage and buffer ph value on the movement of single aunps, we could "image" that positively charged ctab-capped aunps (red) moved faster than negatively charged sds-capped aunps (green), and the former could catch up with and pass the latter. the results visually confirmed the separation rules and some fundamental theories of ce including the effect of buffer ph value on the electroosmotic flow (eof) and the plug-like profile of eof. this novel integration was successfully utilized to evaluate the size distribution and homogeneity of aunps, from which the results obtained were in accordance with transmission electron microscopy (tem) results. the proposed system offers a new insight into ce analysis and has potential applications in many fields, such as the study of interaction between nps and biomolecules in bioanalysis.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000319876800019
公开日期	2014-04-18
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/50267]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Li LB,Yu H,Liu D,et al. A novel dark-field microscopy technique coupled with capillary electrophoresis for visual analysis of single nanoparticles[J]. analyst,2013,138(13):3705-3710.
APA	Li LB,Yu H,Liu D,&You TY.(2013).A novel dark-field microscopy technique coupled with capillary electrophoresis for visual analysis of single nanoparticles.analyst,138(13),3705-3710.
MLA	Li LB,et al."A novel dark-field microscopy technique coupled with capillary electrophoresis for visual analysis of single nanoparticles".analyst 138.13(2013):3705-3710.