Highly sensitive and selective detection of cancer cell with a label-free electrochemical cytosensor

	Highly sensitive and selective detection of cancer cell with a label-free electrochemical cytosensor
	Liu JY ; Qin YN ; Li D ; Wang TS ; Liu YQ ; Wang J ; Wang EK
刊名	biosensors & bioelectronics
	2013
卷号	41 页码:436-441
关键词	ESCHERICHIA-COLI O157-H7 SELF-ASSEMBLED MONOLAYER K562 LEUKEMIA-CELLS GLUCOSE-OXIDASE NANOPARTICLES BIOSENSORS SENSORS IMMOBILIZATION PROLIFERATION IMMUNOSENSOR
ISSN号	0956-5663
通讯作者	liu yq
中文摘要	electrochemical methods have attracted considerable attention for developing cytosensing system since they can decrease the cost and time requirement for cell detection with simple instrumentation. herein, a label-free electrochemical cytosensor with surface-confined ferrocene as signal indicator was developed for highly sensitive and selective detection of cancer cell. with layer-by-layer (lbl) self-assembly technique, positively charged poly(ethylene imine) functionalized with ferrocene (fc-pei) and negatively charged single-wall carbon nanotubes (swnts) were alternately assembled on 3-mercaptopropionic acid (mpa) modified gold substrate. folic acid (fa) was covalently bonded onto swnts surface to specifically recognize cancer cells according to the high affinity of fa for folate receptor (fr) on cellular surface. the developed cytosensor presented high sensitivity and selectivity for the detection of human cervical carcinoma (hela) cell. by using fast-response differential pulse voltammetry (dpv) method, a wide detection range from 10 to 10(6) cells/ml with a detection limit as low as 10 cells/ml was reached even in the presence of a large amount of non-cancerous cells. (c) 2012 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000314191300064
公开日期	2014-04-16
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49974]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Liu JY,Qin YN,Li D,et al. Highly sensitive and selective detection of cancer cell with a label-free electrochemical cytosensor[J]. biosensors & bioelectronics,2013,41:436-441.
APA	Liu JY.,Qin YN.,Li D.,Wang TS.,Liu YQ.,...&Wang EK.(2013).Highly sensitive and selective detection of cancer cell with a label-free electrochemical cytosensor.biosensors & bioelectronics,41,436-441.
MLA	Liu JY,et al."Highly sensitive and selective detection of cancer cell with a label-free electrochemical cytosensor".biosensors & bioelectronics 41(2013):436-441.