Sensitive Detection of Protein Kinase A Activity in Cell Lysates by Peptide Microarray-Based Assay

	Sensitive Detection of Protein Kinase A Activity in Cell Lysates by Peptide Microarray-Based Assay
	Li T ; Liu X ; Liu DJ ; Wang ZX
刊名	analytical chemistry
	2013
卷号	85 期号:15 页码:7033-7037
关键词	GOLD NANOPARTICLE PROBES SIGNAL-TRANSDUCTION FLUORESCENCE PHOSPHORYLATION INHIBITION SPECIFICITY BIOSENSOR FORSKOLIN INSIGHTS BINDING
ISSN号	0003-2700
通讯作者	wang zx
中文摘要	in the present work, the activities of protein kinase a (pka) in cell lysates have been detected by a peptide microarray-based resonance light scattering assay with gold nanoparticle probes. highly sensitive detection of pica activity in 0.1 mu g total cell proteins of shg-44 cell lysate (corresponding to 200 cells) is achieved by a selected peptide substrate. the experimental results also demonstrate that the assay can be employed to evaluate expression levels of pka activity in different cell lines and chemical (e.g., forskolin)-mediated pka activity fluctuation in living cells. in addition, pka inhibition by the inhibitor (h89) is shown, suggesting the potential for screening pka inhibitors at the living cell level.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000323014000011
公开日期	2014-04-15
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49685]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Li T,Liu X,Liu DJ,et al. Sensitive Detection of Protein Kinase A Activity in Cell Lysates by Peptide Microarray-Based Assay[J]. analytical chemistry,2013,85(15):7033-7037.
APA	Li T,Liu X,Liu DJ,&Wang ZX.(2013).Sensitive Detection of Protein Kinase A Activity in Cell Lysates by Peptide Microarray-Based Assay.analytical chemistry,85(15),7033-7037.
MLA	Li T,et al."Sensitive Detection of Protein Kinase A Activity in Cell Lysates by Peptide Microarray-Based Assay".analytical chemistry 85.15(2013):7033-7037.