GALACTURONOSYLTRANSFERASE-LIKE5 Is Involved in the Production of Arabidopsis Seed Coat Mucilage

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 植物代谢工程团队

	GALACTURONOSYLTRANSFERASE-LIKE5 Is Involved in the Production of Arabidopsis Seed Coat Mucilage
	Kong, Yingzhen 1; Zhou, Gongke 4; Abdeen, Ashraf A.5; Schafhauser, James 6; Richardson, Beth 2; Atmodjo, Melani A.1; Jung, Jiyoung 3; Wicker, Louise 3; Mohnen, Debra 1; Western, Tamara 5
刊名	PLANT PHYSIOLOGY
	2013-11-01
卷号	163 期号:3 页码:1203-1217
中文摘要	The function of a putative galacturonosyltransferase from Arabidopsis (Arabidopsis thaliana; At1g02720; GALACTURONOSYLTRANSFERASE-LIKE5 [AtGATL5]) was studied using a combination of molecular genetic, chemical, and immunological approaches. AtGATL5 is expressed in all plant tissues, with highest expression levels in siliques 7 DPA. Furthermore, its expression is positively regulated by several transcription factors that are known to regulate seed coat mucilage production. AtGATL5 is localized in both endoplasmic reticulum and Golgi, in comparison with marker proteins resident to these subcellular compartments. A transfer DNA insertion in the AtGATL5 gene generates seed coat epidermal cell defects both in mucilage synthesis and cell adhesion. Transformation of atgatl5-1 mutants with the wild-type AtGATL5 gene results in the complementation of all morphological phenotypes. Compositional analyses of the mucilage isolated from the atgatl5-1 mutant demonstrated that galacturonic acid and rhamnose contents are decreased significantly in atgatl5-1 compared with wild-type mucilage. No changes in structure were observed between soluble mucilage isolated from wild-type and mutant seeds, except that the molecular weight of the mutant mucilage increased 63% compared with that of the wild type. These data provide evidence that AtGATL5 might function in the regulation of the final size of the mucilage rhamnogalacturonan I.
英文摘要	The function of a putative galacturonosyltransferase from Arabidopsis (Arabidopsis thaliana; At1g02720; GALACTURONOSYLTRANSFERASE-LIKE5 [AtGATL5]) was studied using a combination of molecular genetic, chemical, and immunological approaches. AtGATL5 is expressed in all plant tissues, with highest expression levels in siliques 7 DPA. Furthermore, its expression is positively regulated by several transcription factors that are known to regulate seed coat mucilage production. AtGATL5 is localized in both endoplasmic reticulum and Golgi, in comparison with marker proteins resident to these subcellular compartments. A transfer DNA insertion in the AtGATL5 gene generates seed coat epidermal cell defects both in mucilage synthesis and cell adhesion. Transformation of atgatl5-1 mutants with the wild-type AtGATL5 gene results in the complementation of all morphological phenotypes. Compositional analyses of the mucilage isolated from the atgatl5-1 mutant demonstrated that galacturonic acid and rhamnose contents are decreased significantly in atgatl5-1 compared with wild-type mucilage. No changes in structure were observed between soluble mucilage isolated from wild-type and mutant seeds, except that the molecular weight of the mutant mucilage increased 63% compared with that of the wild type. These data provide evidence that AtGATL5 might function in the regulation of the final size of the mucilage rhamnogalacturonan I.
学科主题	能源植物资源
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	BIOSYNTHETIC HOMOGALACTURONAN GALACTURONOSYLTRANSFERASE ; CELL-ADHESION ; GENE ENCODES ; RHAMNOGALACTURONAN-I ; TRANSCRIPTION FACTOR ; XYLOGLUCAN BIOSYNTHESIS ; PECTIC POLYSACCHARIDES ; FUNCTIONAL-ANALYSIS ; CELLULOSE SYNTHESIS ; XYLAN BIOSYNTHESIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000326520900011
公开日期	2014-03-21
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1582]
专题	青岛生物能源与过程研究所_植物代谢工程团队
作者单位	1.Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA 2.Univ Georgia, Dept Plant Biol, Athens, GA 30602 USA 3.Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 5.McGill Univ, Dept Biol, Montreal, PQ H3A 1B1, Canada 6.McGill Univ, Dept Microbiol & Immunol, Montreal, PQ H3G 1A4, Canada
推荐引用方式 GB/T 7714	Kong, Yingzhen,Zhou, Gongke,Abdeen, Ashraf A.,et al. GALACTURONOSYLTRANSFERASE-LIKE5 Is Involved in the Production of Arabidopsis Seed Coat Mucilage[J]. PLANT PHYSIOLOGY,2013,163(3):1203-1217.
APA	Kong, Yingzhen.,Zhou, Gongke.,Abdeen, Ashraf A..,Schafhauser, James.,Richardson, Beth.,...&Hahn, Michael G..(2013).GALACTURONOSYLTRANSFERASE-LIKE5 Is Involved in the Production of Arabidopsis Seed Coat Mucilage.PLANT PHYSIOLOGY,163(3),1203-1217.
MLA	Kong, Yingzhen,et al."GALACTURONOSYLTRANSFERASE-LIKE5 Is Involved in the Production of Arabidopsis Seed Coat Mucilage".PLANT PHYSIOLOGY 163.3(2013):1203-1217.